与南瓜矮生基因连锁的分子标记研究

本研究以世界上首次发现的中国南瓜矮生突变体（矮10）为供体亲本，以印度蔓生南瓜（蒙日南瓜）为轮回亲本，经6代回交2代自交选育出S1—S9共9个南瓜矮生近等基因纯合株系，其中S2株系再与蒙日南瓜杂交获得F2代分离群体共306株。利用RAPD技术, 采用近等基因系（Near isogenic lines, NILs）分析法，对上述材料进行分析。在640条随机引物中，发现RAPD标记S1225-548与矮生单基因D呈现连锁关系，遗传距离为2.29cM。并且本研究进一步将此RAPD标记成功转化成更为稳定的SCAR标记SCAR3-398。

Research of Molecular Markers Linked to the Dwarf Gene in Squash

In our study, the first discovered dwarf mutant（Ai 10）of the C.moschata Duch was used as the offerer parent, and the normal C .maxima Duch (MengRi) was used as recurrent parent. Nine pure lines of the NILs (Near isogenic lines) named S1—S9 was got through the 6 times backcrosses and twice selfcrosses. The 306 F2 individuals derived from the single cross between the NIL S2 and MengRi. All of the 640 random primers were screened for the marker linked to the dwarf gene D using RAPD and NILs approaches. The results show that the RAPD marker S1225-548 is linked to the dwarf gene. The genetic distance is 2.29cM, and it has been transformed to SCAR marker SCAR3-398.