Comparison of spawning induction techniques on <Emphasis Type="Italic">Paracentrotus lividus</Emphasis> (Echinodermata: Echinoidea) broodstock |
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Authors: | João Gago Orlando J Luís |
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Institution: | 1.Faculdade de Ciências, Centro de Oceanografia—Laboratório Marítimo da Guia,Universidade de Lisboa,Cascais,Portugal |
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Abstract: | The performance of different and expedite spawning induction methods was compared on samples of both mature wild and captive
Paracentrotus lividus sea urchin populations. Thermal, saline and mechanical shocks, emersion during different periods of time, addition of con-specific
gametes and different KCl concentrations were tested. Percentage of spawned sea urchins, mean number of released eggs, percentage
of males that released more than 200 million spermatozoa, and survival after 5 days were the variables analysed. Results indicate
that both thermal and saline shocks were ineffective methods to trigger spawning. Mechanical shock and addition of con-specific
gametes were able to promote spawning but with a reduced number of released gametes. Emersion for a period of 3 h induced
spawning with 100% broodstock survival but longer periods up to 12 h, although increasing the number of spawners can cause
significant broodstock mortality. An injection of 1-mL intra-peristomial KCl was an expedite method to obtain P. lividus gametes, but high mortalities were always recorded and was related with excessive KCl concentrations. When there is need
for a small number of gametes, the mechanical shock technique can be considered since led to 100% survival. When large spawnings
are required, the emersion procedure can be a viable method, but further investigation must be carried out to assess the best
time period to obtain broodstock full survival. |
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