|
|||||
|
|
An assessment of the clonality of the components of canine mixed mammary tumours by mitochondrial DNA analysis |
|
| Authors: | Bertagnolli Angélica C Soares Paula van Asch Bárbara Amorim António Cirnes Luis Máximo Valdemar Cassali Geovanni D |
| Affiliation: | aLaboratory of Comparative Pathology, Department of General Pathology, Institute of Biological Science, Federal University of Minas Gerais, Brazil;bInstitute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal;cDepartment of Pathology, Medical Faculty of Porto, University of Porto, Porto, Portugal;dFaculty of Sciences, University of Porto, Porto, Portugal |
| Abstract: | The aim of this study was to investigate if mutations in the mitochondrial DNA (mtDNA) D-loop fragment control region of canine mammary mixed tumours could be used as clonal markers that identified the cell population of origin. Ten benign mixed mammary tumours and nine carcinomas arising from benign mixed tumours were microdissected and DNA from epithelial and mesenchymal tumour cells and from normal mammary tissue was examined for sequence variations in a fragment of the hypervariable control region.Identical sequence variants in both the epithelial and mesenchymal components (as well as in the corresponding normal tissue) were found in 80% of the benign mixed tumours and in 89% of the carcinomas arising from benign mixed tumours suggesting a shared clonal origin. The distinctive sequence alterations identified in the epithelial and mesenchymal components of 15.8% of all 19 tumours examined, suggests the possibility that a minority of mammary tumours are polyclonal in origin or that early clonal divergence occurs. Increased mutation within the mtDNA D-loop fragment of mixed tumour components was not observed. |
| Keywords: | Benign mixed mammary tumour Clonality Carcinoma D-loop Myoepithelial cell |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |