| RNA干涉培育低木质素杨树 |
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| 引用本文： | 宋恩慧,蔡诚,魏国,高慧,项艳.RNA干涉培育低木质素杨树[J].林业科学,2010,46(2). |
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| 作者姓名： | 宋恩慧 蔡诚 魏国 高慧 项艳 |
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| 作者单位： | 安徽农业大学,生物质改良与转化省级重点实验室,合肥,230036 |
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| 基金项目： | 安徽省2008年科技攻关计划重大科技专项(08010302073);;安徽省教育厅重点项目(KJ2008A128);;国家“十一五”科技支撑项目(2006BAD03A1505) |
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| 摘 要： | 采用改良的CTAB法提取南林95杨的基因组DNA,经PCR扩增得到肉桂酰辅酶A还原酶CCR基因的第4个外显子部分序列,通过中间载体pUCCRNAi,构建含正反向干涉片段的pBI121表达载体,导入农杆菌LBA4404。利用叶盘法侵染南林95杨,获得3株转基因植株,经分子鉴定证实干涉片段已导入南林95杨。测定Klason木质素及综纤维素含量的结果显示:转基因植株Klason木质素含量与对照相比平均降低了9.86%,综纤维素含量与对照相比平均增加了3.17%,纤维长宽比明显增加,均表明转基因植株更有利于造纸。
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| 关 键 词： | CCR RNAi 木质素 遗传转化 |
Cultivation of Low Lignin Poplar by RNA Interference |
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| Song Enhui,Cai Cheng,Wei Guo,Gao Hui,Xiang Yan.Cultivation of Low Lignin Poplar by RNA Interference[J].Scientia Silvae Sinicae,2010,46(2). |
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| Authors: | Song Enhui Cai Cheng Wei Guo Gao Hui Xiang Yan |
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| Institution: | Key Laboratory of Provincial Biomass Improvement and Conversion Anhui Agricultural University Hefei 230036 |
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| Abstract: | Total DNA of 'Nanlin 95' poplar was isolated by CTAB method with adjustment,and the fourth extron fragment(340 bp) from CCR genome amplified by using PCR methods.The PCR products were subcloned into pUCCRNAi with the forward and reverse primers,and transferred into pBI121 expression vector.The expression vector sequence of CCR,were obtained and approved by molecular identification.In comparison with the control,Klason lignin content of transgenic plants reduced 9.7 percent,holocellulose content increased by 3.14 percent,and the fiber aspect ratio remarkably increased.The transgenic plants are better material for pulp making. |
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| Keywords: | CCR RNA interference lignin genetic transformation |
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