耳叶补血草花粉萌发及花粉管生长状况的荧光显微观察

目的]耳叶补血草是蓝雪科补血草属的多年生草本植物,具有较高的生态、药用和观赏价值.但其结实率极低影响了种群的繁殖.方法]通过野外观测、荧光染色等手段对其花部特征、花粉萌发及花粉管生长状况等进行研究,以探讨耳叶补血草在传粉、受精过程中影响种子形成的因素.结果]耳叶补血草的单花寿命较短仅1d,开花和散粉都集中在早上；花柱5枚,柱头丝状,但柱头花粉数量少且自花粉滞溜严重.自然状况下,花粉落置在柱头上5h后开始萌发,10 h时花粉管长约为花柱长度的1/4,20 h时花粉管达到花柱的2/3处,25～30 h花粉管到达子房.异花授粉比自花授粉有着较高的花粉萌发率和花粉管生长速度.结论]耳叶补血草较多的花柱数目并没有增强雌蕊的授粉能力.柱头较差的花粉粘着力、自花粉滞留严重,且挤占花柱通道而抑制异花粉的萌发和生长,成为耳叶补血草传粉受精方面影响结实的因素.

The Observation of Pollen Germination and Pollen Tube Growth in Limonium otolepis by Fluorescence Microscopy

Objective]Limonium otolepis L.is a perennial herb in Plumbaginaceae Limonium Mill and has high ecological,medicina and ornamenta value.However,its low fruit - set limits its population reproduction.Method]The floral traits,pollen germination and pollen tube growth conditions of L.otolepis L were investigated by outdoor observation at fixed - spot,fluorescence staining and other methods were used to explore the factors related to seed setting in the process of pollination and fertilization.Result]The resu...