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| 抗逆相关基因GmDREB导入农杆菌的研究 | |
| 引用本文： | 王萍,高世庆,郭永来,程宪国,王罡,马有志.抗逆相关基因GmDREB导入农杆菌的研究[J].吉林农业大学学报,2007,29(2):148-151. |
| 作者姓名： | 王萍 高世庆 郭永来 程宪国 王罡 马有志 |
| 作者单位： | 1. 淮海工学院海洋学院,连云港,222005 2. 中国农业科学院作物科学研究所,北京,100081 3. 长春龙洋大豆生物蛋白股份有限公司,长春,130041 4. 中国农业科学院资源与农业区划研究所,北京,100081 5. 天津大学农业与生物工程学院,天津,300072 |
| 基金项目： | 教育部大豆生物学重点实验室资助项目 , 国家转基因植物研究和产业化专项基金 |
| 摘 要： | 利用改进的冻融法和电击法成功地将带有编码DREB转录因子GmDREB基因的prdGm-200双元载体分别导入根癌农杆菌EHA101、EHA105和LBA4404菌株中,同时探讨了影响冻融法和电击法转化效率的因素。结果表明:农杆菌细胞OD600约为0.6时冻融法和电击法的转化效率均较高。在利用冻融法转化农杆菌时,新制备的感受态细胞有利于提高转化效率。在进行电击法转化农杆菌时,电场强度和脉冲时间都对农杆菌的转化效率有影响。 |
| 关 键 词： | 根癌农杆菌 GmDREB基因 转化 |
| 文章编号： | 1000-5684（2007）02-0148-04 |
| 修稿时间： | 2006-04-262006-07-16 |
Study on Transformation of Stress Resistance Related Gene GmDREB into Agrobacterium tumefaciens |
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| WANG Ping, GAO Shi-qing, GUO Yong-lai, CHENG Xian-guo, WANG Gang,et al..Study on Transformation of Stress Resistance Related Gene GmDREB into Agrobacterium tumefaciens[J].Journal of Jilin Agricultural University,2007,29(2):148-151. | |
| Authors: | WANG Ping GAO Shi-qing GUO Yong-lai CHENG Xian-guo WANG Gang |
| Institution: | 1. School of Marine Science and Techology , Huaihai Institute of Technology, I~anyungang 222005, China ; 2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; 3. Changchun Longyang Soybean Biological Albumen Co. Ltd. , Changchun 130041, China ; 4. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 5. College of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China |
| Abstract: | The binary vector prdGM-200 with GmDREB gene coding for translation factor DREB was transformed into Agrobacterium tumefaciens strains EHA101,EHA105 and LBA4404 by using freeze-thaw method and electroporation respectively.The factors influencing transformation efficiencies through freeze-thaw and electroporation methods were studied.The results showed that higher transformation efficiency was observed when the value of OD600 reached 0.6.The new competent cell could increase transformation efficiency using freeze-thaw method.While transformation efficiency was influenced by electric field intensity and pulse time when electroporation method was applied. |
| Keywords: | Agrobacterium tumefaciens GmDREB gene transformation |
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