母鼠围产期铅暴露对仔鼠组织微量元素含量的影响及乙酰半胱氨酸的保护效应

为观察母鼠围产期铅暴露对仔鼠各组织微量元素含量的影响及N-乙酰半胱氨酸(NAC)的保护效应,采用电感耦合等离子体质谱法(ICP-MS)测定低水平铅暴露后的仔鼠血液、大脑、骨骼、肝脏和肾脏等组织中的铅、锌、铜、铁、锰和硒等微量元素的含量。结果表明,与对照组相比,染铅组所测组织铅含量均极显著升高(P<0.01),锌含量均极显著降低(P<0.01);肝脏铁含量、肾脏硒含量及骨骼铁、铜、硒含量均显著降低(P<0.01或P<0.05)。NAC拮抗组与染毒组比较,各组织铅含量显著降低(P<0.01或P<0.05),血液和肾脏锌含量显著升高(P<0.05),其他元素含量无明显差异(P>0.05)。

The impact of perinatal exposure to lead in female rats on trace element contents in weaning rat and protective effect of N-acetylcysteine

This study was performed to investigate the contents of trace elements in weaning rat induced by perinatal exposure to lead in female rats,as well as the protective effect of N-acetylcysteine(NAC).The inductively couple plasma-mass spectrometry(ICP-MS) was applied to measure the contents of trace elements(lead,zinc,copper,iron,manganese,selenium) in the blood,brain,ossature,liver and kidney in weaning rats.The results showed that,in comparison with control group,the content of lead in the tissues of lead exposure group increased significantly(P<0.01);the content of zinc decreased significantly(P<0.01);the contents of iron in the liver,selenium in the kidney and iron,copper,selenium in the ossature decreased significantly(P<0.01 or P<0.05).Compared with lead exposure group,the content of lead in the tissues of NAC group decreased significantly(P<0.01 or P<0.05),and the content of zinc increased significantly in the serum and kidney(P<0.05),but other trace elements had no obvious changes(P>0.05).