水稻窄叶突变体nal 11的遗传分析

窄叶突变体的遗传分析,为其基因定位和水稻株型育种提供参考。经甲基磺酸乙酯诱导泸恢17获得的窄叶突变体(nal 11),分别与绵恢727、R30和辐恢838等3个亲本正反交,构建F2群体,考察抽穗期nal 11和绵恢727功能叶的叶宽和叶长,并进行nal 11遗传分析。结果表明:nal 11剑叶长度比杂交亲本绵恢727略短,差异显著,其倒二叶和倒三叶长度则与亲本无差异;该突变体3片功能叶宽度分别仅为绵恢727的63%、61%和79%,明显窄;该突变体与3个杂交亲本正反交,F_1叶片均表现正常宽度,6个F2群体均发生性状分离,正常和窄叶植株比例均符合3:1,说明nal 11是细胞核单基因隐性突变,研究结果为其定位奠定基础。

Genetic Analysis of a Narrow Leaf Mutant Narrow Leaf 11 (nal 11) in Rice (Oryza sativa L.)

Genetic analysis of narrow leaf mutant may provide a reference for gene mapping and plant-shape breeding in rice.A narrow leaf mutant (nal 11) was induced by ethyl methyl sulfonate from Luhui 17.nal 11 was crossed reciprocally with Mianhui 727,R30 and Fuhui 838,respectively,to construct F2 populations.The length and width of the three functional leaves of nal 11 and Mianhui 727 were investigated at heading stage and the genetic analysis of nal 11 was also carried out.The results showed that:Length of the flag leaf of nal 11 was significantly shorter than cross-parent of Mianhui 727;length of the 2nd and 3rd leaf from the top were not different between nal 11 and Mianhui 727.However,width of the three functional leaves of the nal 11 was only 63％,61％ and 79％ of the width of Mianhui 727,respectively.When crossed reciprocally with three parents,the leaf width of F1 was normal.Trait segregation of leaf width occurred in all of the six F2 populations.The ratios of normal and narrow leaf plants accorded with 3:1,which suggested that nal 11 was controlled by a single recessive nuclear gene.These results lay a foundation for localization of the gene.