高产絮凝物蝉花菌株筛选和絮凝条件初探

为探究虫生真菌蝉花不同菌株之间产絮凝剂的差异及絮凝物的絮凝特性,以高岭土法对36株蝉花菌株进行了高产絮凝物的筛选及高产菌株的生长特性和絮凝性质的研究。实验筛选出稳定、高产絮凝物的蝉花菌株GZUIFR-6722,絮凝物活性物质是其胞外聚合物。该菌株在生长过程中生物量的积累和产絮凝物能力呈正相关。当菌株6 722絮凝物投加量为1.2 mL每50 mL时,絮凝效果较好,絮凝率可达91.47%。1%的CaCl_2能显著促进絮凝效果,絮凝率可达94.89%。颠倒次数、静置时间、温度以及pH对絮凝效果影响不显著。

Screening of High Bioflocculability Strain of Isaria cicadae Mig and the Flocculating Conditions

The aim of this paper is to study the bioflocculability and characteristics of different strains of Isaria cicadae by screening all 36 strains with kaolin.The results showed that GZUIFR-6722 strain can perform steadily with high bioflocculability.The active substrate was identified as some kind of extracellular metabolite.During the growth,the production of active substrate was related positively with the biomass.The flocculating rate was 91.47% after addition of 1.2mL/50mL bioflocculants.Moreover,the addition of 1% CaCl2 could promote the performance of the bioflocculants,and increased the flocculating rate to 94.89%.Furthermore,the results also indicated that inversion,standing time,temperature and pH had no obvious effect on flocculation.The findings suggest for application of the GZUIFR-6722 strain in the treatment of waste water.