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连翘内生菌的分离及对植物病原菌的抑菌活性测定
引用本文:冀玉良,李丹青,李堆淑,何军.连翘内生菌的分离及对植物病原菌的抑菌活性测定[J].西南农业学报,2011,24(6).
作者姓名:冀玉良  李丹青  李堆淑  何军
作者单位:1. 商洛学院生物医药工程系,陕西商洛,726000
2. 商洛学院化学与化学工程系,陕西商洛,726000
基金项目:陕西省教育厅科学技术研究项目(08JK280)
摘    要:采用组织块分离法,从药用植物连翘的报、茎、叶中分离出内生菌136株,其中真菌71株,细菌42株,放线菌23株;以烟草赤星菌,苹果干腐病,棉立枯病菌,茄腐皮病镰孢霉,革燕麦镰孢霉5种植物病原菌作为靶标菌,测定了连翘内生菌的抗菌活性.结果表明连翘中有比较丰富的具有抗菌活性的内生菌,并初步筛选出了9株抗菌活性高的内生真菌、7株内生细菌和4株内生放线菌.

关 键 词:连翘  内生菌  抑菌活性

Isolation of Endophyte from Fructus forsythiae and Determination of Its Inhibition Activities to Plant Pathogens
JI Yu-liang , LI Dan-qing , LI Dui-shu , HE Jun.Isolation of Endophyte from Fructus forsythiae and Determination of Its Inhibition Activities to Plant Pathogens[J].Southwest China Journal of Agricultural Sciences,2011,24(6).
Authors:JI Yu-liang  LI Dan-qing  LI Dui-shu  HE Jun
Institution:JI Yu-liang1,LI Dan-qing2,LI Dui-shu1,HE Jun1(1.Biological and Medical Engineering Department of Shangluo University,Shanxi Shangluo 726000,China,2.Chemistry & Chemical Engineering Department of Shangluo University,China)
Abstract:136 strains of entophytes were isolated from roots,stems and leaves of Fructus forsythiae with tissue isolation method,in which 71 strains were entophytic fungi,42 strains were entophytic bacteria and 23 strains were entophytic actinomycetes.Iinhibition activities of the entophytes was measured with Alternaria longipes Tobacco pathotype,Botroyosphaeria ribis,Rhizoctonia solani,Fusarium solani(Mart.)App.et Wollenw and Fusarium avenaceum var.herbarum(Corda) Sacc 5 species of plant pathogens as a target.The re...
Keywords:Fructus forsythiae  Endophyte  Inhibition activities  
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