产油单针藻的最适培养基筛选及产油特性提升

为了研究培养基种类及成分对单针藻产油特性的影响,充分挖掘单针藻产油潜能,以Monoraphidium sp. HDMA-11为供试藻株,以细胞数监测、干重法和尼罗红染色法检测了BG-11培养基、BBM培养基、TAP培养基、D1培养基和SE培养基对藻株生长速度、生物量及含油量的影响,并对藻株可利用的碳源、氮源种类及浓度进行筛选与优化。结果表明,TAP培养基可极大缩短藻株培养周期,使藻株生物量生产率提升了127%,油脂生产率提升了117%。当乙酸添加量为2 mg/L,氯化铵浓度为750 mg/L,藻株的产油指标进一步提高。优化后藻株的生物量生产率为70 mg/(L·d),较初始培养条件提升了218%,优化后藻株的油脂生产率为315 RFU/(L·d),较初始培养条件提升了116%。本研究优化了Monoraphidium sp. HDMA-11的培养条件,明确了乙酸和氯化铵浓度对藻株生长特性及产油指标的影响,为进一步挖掘藻株产油潜能提供了数据支持。

Lipid-producing Monoraphidium sp.: The Best Medium Screening and Lipid-producing Property Enhancement

To investigate the effects of medium type and composition on the lipid-producing properties of Monoraphidium sp. HDMA-11 and fully improve the lipid production potential of the strain, the growth, biomass and lipid content of HDMA-11 were examined using cell counting, dry cell weight and Nile red staining methods in BG-11, BBM, TAP, D1 and SE media. The type and concentration of carbon and nitrogen sources were then optimized to improve the lipid production characteristics of the strain. Results showed that the TAP medium could greatly shorten the culture period of the strain and increase its biomass productivity by 127% and lipid productivity by 117%. The lipid-producing indexes of the strain were further improved when 2 mL/L of acetic acid and 750 mg/L of ammonium chloride were added. The productivity of biomass and lipid of the strain after being improved were 70 mg/(L·d) and 315 RFU/(L·d) respectively, which was 218% and 116% higher than those of the initial levels. This work optimized the culture conditions of HDMA-11, elucidated the effects of acetic acid and ammonium chloride on the growth and lipid-producing indexes, and provided data basis for further exploration of the lipid-producing potential of the strain.