唐菖蒲伯克霍尔德菌产毒差异菌株菌体脂肪酸分析

为了探讨脂肪酸与椰毒假单胞菌米酵菌酸毒素产生之间的相关性，采用气相色谱和面积归一法分别对产毒差异菌株的菌体脂肪酸进行分离和定量分析。结果显示，9株菌株共分离到11种组分，其中十六碳酸(C16:0）、顺-10-十七碳一烯酸(C17:1)以及顺,顺-9,12-十八碳二烯酸(C18:2n6c)含量较高，平均含量依次为43.85%、25.84%和14.75%。培养基及培养温度是影响脂肪酸组成的主要因素，相同培养条件下，9株菌株菌体脂肪酸组成相似度达90%以上。毒性试验后，不产毒菌株10574与产毒菌株菌体脂肪酸均发生相似变化，即顺-9-十八碳一烯酸(C18:1n9c)含量降低，而顺,顺-9,12-十八碳二烯酸(C18:2n6c)含量升高。生长条件是影响菌体脂肪酸组成的主要因素，脂肪酸与椰毒假单胞菌米酵菌酸产生的相关性不大。

Analysis of Fatty Acids in Different Strains of Burkholderia gladioli

In order to explore the correlation between fatty acids and the production of bongkrekic acid from Pseudomonas cocovenenans subsp. farinofermentans, the fatty acids of different toxin-producing strains were separated by gas chromatography, and the chromatographic peaks were quantitatively analyzed by area normalization method. The results showed that a total of 11 components were isolated from 9 strains, including hexadecanoic acid (C16:0), cis-10-heptadecaenoic acid (C17:1) and cis,cis-9,12-octadecadienoic acid (C18:2n6c), with an average content of 43.85%, 25.84% and 14.75%, respectively. Culture medium and culture temperature were the main factors affecting fatty acid composition. Under the same culture conditions, the similarity of fatty acid composition of the 9 strains was more than 90%. After the toxicity test, the fatty acids of non-virulent strain 10574 and toxin-producing strain showed similar changes, the content of cis-9-octadecenoic acid (C18:1n9c) decreased, while the content of cis,cis-9,12-octadecadienoic acid (C18:2n6c) increased. The culture condition is the main factor affecting the fatty acid composition of Pseudomonas cocovenenans subsp. farinofermentans, and there is little correlation between fatty acids and the production of bongkrekic acid.