利用SRAP和RGA标记对新疆海岛棉品种的聚类分析

目的]对新疆自育的新海系列品种进行遗传多样性研究.方法]利用SRAP和RGA标记,检测出多态性位点；利用NTSYSpc2.1软件,分别计算两种分子标记数据的系数矩阵,采用UPGMA法对所选材料进行聚类分析.结果]SRAP共检测出了134个多态性位点,每组合的多态性条带数从2～8不等,平均每个引物组合产生2.91个多态性位点,表明SRAP能检测出较多的遗传位点,能够较好地反映海岛棉的遗传多样性.RGA标记共扩增出了46个多态性位点,每组合的多态性条带数从2～5不等,平均每个引物组合产生2.08个多态性位点,表明RGA作为一种分子标记也能检测出较多的遗传位点,能够较好地反映海岛棉在抗病方面的遗传多样性.结论]SRAP聚类结果基本与品种系谱来源一致,RGA聚类基本与材料的抗病水平一致.分子标记在鉴别品种和品种遗传多样性研究方面具有重要作用.

Cluster Analysis on Xinjiang Sea-Island Cotton by SRAP and RGA

【Objective and Method】 The sea-island cotton(Gossypium barbadense) is important due to its fine fiber quality.However,genetic diversity analysis of this species is still poorly documented,which is important for in situ and ex situ germplasm conservation and useful for breeding programs.Genetic diversity of sea-island cotton cultivars from Xinjiang were assessed by SRAP and RGA markers.Jaccard coefficient matrix of two molecular marker data were calculated respectively by NTSYSpc2.1 software and clustering analysis was made by using UPGMA method on all selected materials.【Result】Using 238 SRAP markers,134 polymorphism loci were detected.The number of polymorphic loci per primer ranged from 2 to 8,with an average of 2.91 which showed that more polymorphism loci can be detected and the genetic diversity of sea-island cotton can be reflected better by using SRAP.Using 107 RGA markers,46 polymorphism loci were detected.The number of polymorphic loci per primer ranged from 2 to 5,with an average of 2.08 which showed that more polymorphism loci also can be detected and the resistance genetic diversity of sea-island cotton can be reflected better by using RGA as a molecular marker.【Conclusion】The results showed that the SRAP clustering was basically consistent with pedigree source and the RGA clustering was basically consistent with resistance level of the materials.The molecular marker was proved to play an important role on host identification and genetic diversity study of varieties.