| 枫叶秋海棠组织培养与快繁技术的研究 |
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| 引用本文： | 宗宪春,宗灿华. 枫叶秋海棠组织培养与快繁技术的研究[J]. 安徽农业科学, 2010, 38(24): 13561-13562 |
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| 作者姓名： | 宗宪春 宗灿华 |
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| 作者单位： | 1. 牡丹江师范学院生命科学与技术学院,黑龙江牡丹江,157012
2. 牡丹江医学院,黑龙江牡丹江,157012 |
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| 摘 要： | [目的]通过组织培养方法筛选枫叶秋海棠不定芽诱导、伸长及生根的最佳培养基，为枫叶秋海棠的快速繁殖提供参考。[方法]以枫叶秋海棠的叶柄、叶片为外植体，进行不定芽诱导、伸长及生根的组织培养。[结果]枫叶秋海棠叶片最佳芽诱导、芽伸长培养基为MS＋zr（2．0mg／L）＋NAA（0．2mg／L）。生根的最佳培养基为1／2MS＋IBA（0．2mg／L），试管苗移栽后成活率为100％。[结论]该试验建立了枫叶秋海棠组织培养快速繁殖体系，为枫叶秋海棠的快速繁殖和遗传转化提供理论依据。
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| 关 键 词： | 枫叶秋海棠 组织培养 快速繁殖 |
Study on the Rapid Propagation with Tissue Culture of Begonia heracleifoniana |
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| ZONG Xian-chun et al. Study on the Rapid Propagation with Tissue Culture of Begonia heracleifoniana[J]. Journal of Anhui Agricultural Sciences, 2010, 38(24): 13561-13562 |
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| Authors: | ZONG Xian-chun et al |
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| Affiliation: | ZONG Xian-chun et al (College of Life Science and Technology,Mudanjiang Teachers College,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | [Objective] The study aimed to screen the optimum medium for the induction elongation of adventitions bud,and rooting of Begonia heracleifoniana and provide reference for the rapid propagation of Begonia heracleifoniana.[Method] With leaves and petiole of Begonia heracleifolia as explants,the culture condition of the induction of adventitions bud and rooting were studied.[Result] The optium medium for the differentiation and elongation of leaves from Begonia heracleifonia was MS+ZT(2.0 mg/L)+NAA(0.2 mg/L).T... |
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| Keywords: | Begonia heracleifoniana Tissue culture Rapid propagation |
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