黄瓜营养体苦味基因Bi的定位

以黄瓜（Cucumis sativus L.）营养体无苦味的材料9110Gt（bibi）与有苦味的材料9930（BiBi）为亲本，对以两亲本构建的148个株系的RILs群体进行SSR标记的遗传连锁分析，对Bi基因进行初步定位，两侧翼标记SSR19672和SSR00004与Bi基因的连锁距离分别为4.4和3.4 cM。利用两侧翼标记筛选以相同亲本构建的1 815株的F2群体中的重组单株，结合黄瓜基因组测序的结果及生物信息学的知识，在标记区域内开发了31对SSR标记，最终将Bi基因定位在黄瓜的第6染色体上，最近的两侧翼标记SSR02309和SSR00004与苦味基因分别相距1.7和2.2 cM。

Fine Mapping of the Foliage Bitterness Gene (Bi) in Cucumis sativus

A RILs population with 148 individuals derived from a cucumber line 9110 Gt（bibi）and 9930（BiBi）was used to analyze the location of Bi gene. Bi gene was mapped on the linkage group 6, corresponding to chromosome 6 of cucumber. The flanking markers SSR19672 and SSR00004 were linked to the Bi gene with distances of 4.4 and 3.4 cM. An F2 population with 1 815 individuals derived from the same parents was used for screening recombinants for fine mapping of the gene. Recombinant plants were identified by the flanking markers SSR19672 and SSR00004. Based on the whole genome sequence of cucumber，thirty-one SSRs within the region between SSR19672 and SSR00004 were designed and screened for polymorphism between the two parents, which revealed that one of them（SSR02309）was 1.7 cM to Bi gene. The distance between previously identified flanking marker SSR00004 and Bi were 2.2 cM.