Apparent seroprevalence,isolation and identification of risk factors for brucellosis among dairy cattle in Goa,India |
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| Institution: | 1. Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India;2. Goa State Co-Operative Milk Producers’ Union Limited, Curti, Ponda 403 401, India;3. Network Project on Brucellosis, Department of Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India;1. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;3. Rheumatology Research Center, Tehran University of Medical Sciences, Iran;4. Department of Biostatistics, School of Medical Sciences, Tarbiat-Modares University, Tehran, Iran;1. Santé publique France, French Public Health Agency, 94410 Saint-Maurice, France;2. French Agency for Food, Environmental and Occupational Health and Safety (Anses), 94700 Maisons-Alfort, France;3. University Hospital Caremeau, Department of Microbiology, 30000 Nîmes, France;4. University of Montpellier, Institut National de la Santé et de la Recherche Médicale, U1047, 30000 Nîmes, France;5. University Hospital Caremeau, Department of Infectious Diseases, 30000 Nîmes, France;6. University Hospital and University, 38000 Grenoble, France;1. Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, India;2. Immunology Section, Indian Veterinary Research Institute, Izatnagar, India;1. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran;2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran;3. Researcher in the Rheumatology Research Center, Tehran University of Medical Sciences, IR Iran;4. Department of Biostatistics, School of Medical Sciences, Tarbiat-Modares University, Tehran, IR Iran |
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| Abstract: | Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. The disease has been reported worldwide except in few countries where it has been eradicated. The prevalence of brucellosis among cattle from 11 farms having a history of abortions was studied. A total of 481 samples comprising of blood, milk, vaginal swabs, vaginal discharges, placental tissues and fetal tissues were collected from 296 animals. Clinical samples were processed for the isolation of Brucella. Serum samples (n = 296) were tested by Rose Bengal Plate Test (RBPT) and indirect ELISA. A total of 90 (30.40%) and 123 (41.55%) samples were positive by RBPT and indirect ELISA, respectively. Also 27.02% samples were positive by both the tests. Brucella isolates (n = 8) were recovered from clinical samples using Brucella selective media. All the isolates demonstrated PCR amplification for the bcsp31 and IS711 genes. Amplification of Brucella abortus specific primer was demonstrated by all the isolates in AMOS PCR indicating isolates to be of either B. abortus biotype 1, 2 or 4. Risk factors for transmission of brucellosis among cattle population were studied by field surveys. It was observed that lack of awareness about brucellosis (OR = 8.739, P = 0.138) and inadequate floor space (OR = 0.278, P = 0.128) were crucial risk factors for transmission of bovine brucellosis. |
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| Keywords: | Seroprevalence Strain identification AMOS PCR Risk factors |
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