Cis- and trans-protopinium,a novel nematicide,for the eco-friendly management of root-knot nematodes |
| |
| Institution: | 1. Department of Plant Pathology, The University of Agriculture, Peshawar, 25130, Khyber Pakhtunkhwa, Pakistan;2. Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, 25130, Khyber Pakhtunkhwa, Pakistan;3. Department of Poultry Sciences, The University of Agriculture, Peshawar;4. Department of Crop Protection, Institute for Sustainable Agriculture (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Apartado 4084, 14080 Córdoba, Spain;5. Phytopharmaceutical and Nutraceutical Research Lab, Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan;1. School of Information Science and Engineering, Shandong University, Jinan, Shandong 250100, China;2. Key Laboratory for Laser Plasmas (Ministry of Education) and Department of Physics, Shanghai Jiao Tong University, Shanghai 200240, China;3. Key Laboratory of Materials for High Power Laser, Shanghai Institute of Optics and Fine Mechanics, Shanghai 201800, China;4. State Key Laboratory of Transient Optics and Photonics, Xi?an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi?an 710119, China;1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China;2. Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China;3. School of Life Sciences, Yunnan University, Kunming 650091, PR China;1. Department of Agricultural Economics, University of Ibadan, Nigeria;2. Department of Agricultural Economics and Animal Production, University of Limpopo, South Africa;1. State University of Maringa, Dept. of Agrarian Science, Umuarama, PR, Brazil;2. State University of Maringa, Postgraduated in Agricultural Science, Umuarama, PR, Brazil;3. State University of Maringa Dept. of Chemical Engineering, Maringá, PR, Brazil;1. College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China;3. Shandong Institute of Pomology, Tai''an, 271000, China |
| |
| Abstract: | Plant-derived nematicides are being increasingly used while select synthetic nematicides are phased out for environmental concerns. This is the first report on the in vitro nematicidal activity of cis- and trans-protopinium isolated from roots of Fumaria parviflora and its in vivo effect on the southern root-knot nematode Meloidogyne incognita. Cis- and trans-protopinium was isolated from the methanolic fraction FM2.1, and its structure elucidated using 13C and 1H nuclear magnetic resonance (NMR). The NMR spectra were characterized using deuterated dimethyl sulfoxide (DMSO) at temperatures of 25 and 80 °C. In an in vitro study, over 120 h of incubation, the area under cumulative percent hatch inhibition and mortality of cis- and trans-protopinium reached 100% at a concentration of 200 μg mL?1. In the greenhouse and field settings, cis- and trans-protopinium was evaluated against M. incognita on tomato at a concentration of 100, 200, and 300 μg mL?1 for two consecutive seasons, that is, spring and autumn, in 2010. At a concentration of 300 μg mL?1 in the greenhouse and field trials during spring and autumn, cis- and trans-protopinium significantly reduced the nematode galling index, the number of females per gram of root, and the reproduction factor, as well as increased plant height, fresh and dry shoot weights, and root length. Therefore, cis- and trans-protopinium can be used as an effective and safe nematicide against M. incognita on tomato in an organic and sustainable agricultural production system. Phytochemicals have various agricultural applications, especially to control economically important nematode pests. |
| |
| Keywords: | Nematicidal activity Integrated disease management Greenhouse Tomato |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
