秦川牛CS-1 基因SNPs 检测及其与胴体、肉质性状相关性研究

【目的】检测秦川牛CS-1基因的SNPs多态性，并将其与秦川牛胴体和肉质性状进行关联分析。【方法】采用PCR-SSCP方法对485头秦川牛CS-1基因第3外显子进行SNPs多态性检测，分析其与部分胴体、肉质性状的关系; 【结果】在CS-1基因第3外显子191碱基处发现C→A突变，201碱基处发现C→T突变。检测到A和C两个等位基因，其基因频率分别位0.1381和0.8619。方差分析结果表明：胴体性状方面， AC 型个体背部皮下脂肪厚显著高于CC 型个体(P＜0.05)；在肉质性状方面，AC 型个体的大理石花纹显著高于CC 型个体（P<0.05），AA 型和AC 型个体的嫩度要显著优于CC 型个体(P＜0.05)；其余性状差异不显著; 【结论】揭示可以用该多态性位点(CS-1 Exon3) 对秦川牛的胴体性状和肉质性状进行标记辅助选择。

SNPs Detection of CS-1 Gene and Its Relationship with Carcass and Meat Quality Traits in Qinchuan Cattle

【Objective】This research was to detect the polymorphism in exon 3 of CS-1gene and its relationship with some carcass and meat quality traits of Qinchuan cattle.【Method】PCR-SSCP and DNA sequencing methods were employed to reveal the polymorphism of exon 3 and its flanking within CS-1 gene in 485 Qinchuan cattle, and analysing its relationship with some carcass and meat quality traits of Qinchuan cattle;【RESULTS】C→A mutation in 191bp and C→T mutation in 201bp of CS-1 gene exon 3 were found. There were two allele gene A and C in this locus with the corresponding frequencies of 0.1381and 0.8619. Statistical analysis indicated that individuals with genotype AC had superior thickness of back fat and marbling (P<0.05) when compared those with genotype CC; As for the tenderness, animals of AA and AC genotype were more excellent than animals with genotype CC(P＜0.05);【CONCLUSION】It suggested that this locus could be used as a molecular marker in Qinchuan cattle breeding for carcass and meat traits.