| 中国野生葡萄抗霜霉病基因RAPD标记的筛选 |
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| 引用本文： | 张艳艳,张剑侠,王跃进.中国野生葡萄抗霜霉病基因RAPD标记的筛选[J].果树学报,2008,25(6):816-820. |
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| 作者姓名： | 张艳艳 张剑侠 王跃进 |
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| 作者单位： | 农业部西北园艺植物种质资源利用重点开放实验室 陕西省农业分子生物学实验室,西北农林科技大学园艺学院,陕西杨凌712100 |
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| 摘 要： | 以抗病的中国野生华东葡萄(Vitis pseudoreticulata)白河-35-1(Baihe-35-1)与感病的欧洲葡萄佳利酿(Carignane)杂交F2代为试材,从520条随机引物中筛选出21条可以扩增多态性DNA片断的随机引物,从中获得了6个抗霜霉病基因的RAPD标记,对这些标记回收、克隆、测序后,将得到的6个标记分别记录为:S294-369,S202-527,S382-615,S221-677,S416-1224和S390-725。通过分析这6个RAPD标记在F2代植株中的表现,证明这些标记都与霜霉病抗性的基因有连锁关系。这将为葡萄抗霜霉病育种的分子标记辅助选择提供分子依据。
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| 关 键 词： | 中国野生葡萄 霜霉病 抗病基因 RAPD标记 |
Screening the RAPD markers linked to the gene resistant to downy mildew in Chinese wild species of Vitis |
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| ZHANG Yan-yan,ZHANG Jian-xia,WANG Yue-jin.Screening the RAPD markers linked to the gene resistant to downy mildew in Chinese wild species of Vitis[J].Journal of Fruit Science,2008,25(6):816-820. |
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| Authors: | ZHANG Yan-yan ZHANG Jian-xia WANG Yue-jin |
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| Institution: | ZHANG Yan-yan,ZHANG Jian-xia,WANG Yue-jin(Key Laboratory of Horticultural Plant Germplasm Resources Utilization Northwest in China,Ministry of Agriculture,P.R.China,Key Labo-ratory of Agriculture Molecular Biology of Shaanxi Province,Horticultural College,Northwest A & F University,Yangling,Shaanxi 712100 China) |
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| Abstract: | The F2 population derived from the cross between the selection Baihe-35-1,resistant to downy mildew and be-longing to a Chinese wild species of Vitis(Vitis pseudoreticulata) and V.vinifera L.cv.Carignane was used as material for screening the RAPD markers linked to the gene resistant to downy mildew.A total of 520 random primers were used to screen RAPD markers which linked to resistance to downy mildew in Chinese wild Vitis.21 random primers could amplify polymor-phic DNA fragments,among which 6 RAPD marke... |
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| Keywords: | Chinese wild species of Vitis Downy mildew Resistant gene RAPD markers |
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