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| 马传染性贫血病毒弱毒疫苗S2基因在体内变异分析 | |
| 引用本文： | 王帅,王雪峰,林跃智,姜成刚,吕晓玲,赵立平,周建华,曲娟娟.马传染性贫血病毒弱毒疫苗S2基因在体内变异分析[J].中国预防兽医学报,2011,33(6). |
| 作者姓名： | 王帅 王雪峰 林跃智 姜成刚 吕晓玲 赵立平 周建华 曲娟娟 |
| 作者单位： | 1. 东北农业大学资源与环境学院,黑龙江哈尔滨150030;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨150001 2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨,150001 3. 东北农业大学资源与环境学院,黑龙江哈尔滨,150030 |
| 基金项目： | 国家自然科学青年基金，国家十一五重大传染病专项 |
| 摘 要： | 为研究马传染性贫血病毒(EIAV)驴白细胞弱毒疫苗EIAVDLV121的S2基因在马体内的变异规律,本研究选用4匹成年马,其中2匹(#1、#2)接种EIAVDLV121,另外2匹作为对照.免疫后监测马体温、血小板含量以及病毒载量结果显示,免疫马未出现马传染性贫血体征.通过RT-PCR方法检测病毒S2基因在感染马体内不同时期的基因序列,结果显示,免疫马体内EIAVDLV121 S2蛋白的突变主要发生在氨基酸第17位、22位、39位、41位、51位和55位.另外,#1马免疫后70 d以及#2马免疫后第14 d和第28 d检测疫苗毒S2蛋白序列与EIAVDLV121亲缘关系较近,而#1马免疫后第42 d、第112 d和第140 d的疫苗毒S2蛋白序列与EIAVDLV121的致病性亲本强毒株EIAVDN40亲缘关系最近.本研究结果有助于对EIAV及EIAV疫苗株在马体内感染进程的研究. |
| 关 键 词： | 马传染性贫血病毒 S2基因 变异分析 |
Genetic variation of S2 gene of the donkey leukocyte-attenuated vaccine strain of equine infectious anemia virus in experimentally infected horses |
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| WANG Shuai,WANG Xue-feng,LIN Yue-zhi,JIANG Cheng-gang,LV Xiao-ling,ZHAO Li-ping,ZHOU Jian-hua,QU Juan-juan.Genetic variation of S2 gene of the donkey leukocyte-attenuated vaccine strain of equine infectious anemia virus in experimentally infected horses[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(6). | |
| Authors: | WANG Shuai WANG Xue-feng LIN Yue-zhi JIANG Cheng-gang LV Xiao-ling ZHAO Li-ping ZHOU Jian-hua QU Juan-juan |
| Institution: | WANG Shuai1,2,WANG Xue-feng2,LIN Yue-zhi2,JIANG Cheng-gang2,LV Xiao-ling2,ZHAO Li-ping2,ZHOU Jian-hua2*,QU Juan-juan1*(1.College of Resource and Environmental Science,Northeast Agricultural University,Harbin 150030,China,2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| Abstract: | The variation of S2 gene of the equine infectious anemia virus(EIAV) attenuated vaccine adapted to cultivated donkey leukocytes was investigated by analysing virus replicated in experimentally infected horses.Two horses,#1 and #2,were inoculated with EIAVDLV121.Body temperature,viral RNA copy and platelet count of these horses were monitored for 6 months.Furthermore,the S2 gene of EIAVDLV121 was amplified by RT-PCR from plasma at different time points and sequenced.Results of sequence analysis showed that t... |
| Keywords: | equine infectious anemia virus S2 gene variation analysis |
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