基于US区缺失位点猪伪狂犬病病毒疫苗株与野毒株鉴别诊断方法的建立

依据GenBank中公布的猪伪狂犬病病毒全基因组序列,针对gI、gE、28K、TK四个基因序列,设计、合成了5条引物,并对野毒株SA株、gI-/gE-/PRVSA双基因缺失株和Bartha K61疫苗株进行了扩增,得到2061bp、1323bp、801bp和963bp特异性目的条带,其中BarthaK61疫苗株基因组检测最小浓度为136pg/μL。建立了针对野毒株和两种疫苗株的鉴别诊断方法。

Establishment of a Differential Diagnosis Based on US District Deletions of Pseudorabies Virus Vaccine Strains and Field Strains

Based on the pseudorabies virus complete genome published on GenBank five primers were designed and synthesized to the four gene sequences including gI,gE,28K,TK,and the field PRV strain SA,gI-/gE-/PRV SA strain and the vaccine strain Bartha K61 were amplified resulting in 2 061 bp,1 323 bp,801 bp and 963 bp specific target bands,the minimum detection concentration for vaccine strain Bartha K61 genome was 136 pg/μL.The differential diagnosis was established for of the field virus strain and the two vaccine strains.