粉红粘帚霉67-1菌株寄生核盘菌研究

 用诱捕法从海南乐东县菜园土壤中获得一株对核盘菌菌核具有强寄生能力的粉红粘帚霉(Gliocladium roseum)菌株67-1,寄生频率为100%。该菌的PDA平板回接核盘菌菌核,一周后寄生率可达100%。对峙培养发现其对核盘菌有明显的抑菌带。保湿条件下,该菌孢子在24h内成功侵入核盘菌菌核。切片显微观察证明该菌能高效侵染菌核,造成组织溃解。寄生过程中菌核内蛋白组成发生明显变化。这一菌株在22~35℃均能很好生长,菌丝及产孢最适温度为24℃,产孢量大。认为这一菌株具有良好的菌核病生防应用潜力。

Mycoparasitism of Gliocladium roseum 67-1 on Sclerotinia sclerotiorum

G. roseum 67-1, a strain with high pathogenicity to sclerotia of S. sclerotiorum, was isolated from pea rhizosphere soil collected from Ledong, Hainan Province. One hundred percent of sclerotia were parasitized 7 d after they were inoculated with G. roseum 67-1 on PDA plates. Spores of this isolate succeeded in parasitizing sclerotia within 24 h after inoculation. The inhibition band was observed during the dual culture on PDA plates. Microscopic observation of sectioned sclerotia showed that sclerotial tissue collapsed by the parasitism of G. roseum 67-1. Change of protein composition was found in the infected sclerotial tissue. Our results show that this isolate, with ability to grow under wide temperature range and to produce mass spores, is of great potential to control soybean stem rot caused by S. sclerotiorum.