禽致病菌ESBLs和AmpC酶的检测及药敏分析

用全自动微生物鉴定系统(VITEK-32)鉴定了禽分离致病菌,分别进行了β-内酰胺酶(BLA)、超广谱β-内酰胺酶(ESBLs)、AmpC酶的检测,并用试管两倍稀释法测定了各种抗生素对非产酶菌、产ESBLs菌及产AmpC菌的抗菌活性。结果表明,鉴定分离的20株致病菌有大肠埃希菌15株、阴沟肠杆菌1株、铜绿假单胞菌1株、法氏柠檬酸杆菌1株、肺炎克雷伯菌1株及鹑鸡肠球菌1株,其中法氏柠檬酸杆菌、肺炎克雷伯菌及鹑鸡肠球菌系兽医上首次检出。报道所分离的20株致病菌均产β-内酰胺酶,其中产ESBLs 9株,同时产ESBLs和AmpC酶1株。产酶菌株对抗生素的耐药性严重,而抗生素/抑制剂联用能降低药物对细菌的MICs。

Detection of Extended-spectrum β -lactamases and AmpC Enzyme and Antibiotics Susceptibility Test Analysis of Pathogens Isolated from Poultry

The antibacterial activity of various antibiotics or beta-lactams with inhibitors against non-beta-lactamase-prouding,extended-spectrum beta-lactamase(ESBLs)-producing and AmpC enzyme-producing isolates were evaluated with two-fold dilution method after pathogens isolated from poultry were identificated with VITEK-32 and were detected respectively for beta-lactamase,ESBLs and AmpC enzyme.The results revealed that there are 15 Escheria coli,1 Enterobacter cloacae,1 Pseudomonas aeriginosa,1 Citrobater farmeri,1 Klebsiella pneumoniae and 1 Entercoccus gallinarum in the 20 pathogens.In these pathogens,the citrobacter farmeri,Klebsiella pneumoniae and Enterococcus gallinarum were first isolated from poultry in veterinary medicine.All of the isolated strains could produce beta-lactamase,8 strains could produce ESBLs and 1 strains could produce ESBLs and AmpC enzyme.All of the 20 strains isolated were resistant to antibiotics except for the imipenem and meropenem.The combination of beta-lactams with inhibitors could decrease the MICs of antibiotics against isolates.