加速器氩离子辐射对水稻诱变效应的初步研究

用加速高能氩离子束照射水稻干种子,观察M_1的损伤效应,建立了成苗率与育性的剂量响应曲线,并求出成苗率与育性的半抑制剂量分别为93.7Gy及67.0Gy。在M_2选出了具早熟、矮秆、大粒等性状的多个突变体,并发现多种性状同时变异的突变体所占的比例较大,而不同剂量处理的诱发突变效果有较大差异,在所用剂量范围内,以90Gy处理诱发的突变频率最高,突变谱最广。应用同工酶作为遗传标记研究了所观察的性状突变与9种同工酶酶谱的关系。结果表明,在分析的突变体中,有两种同工酶(PGD-1及PGI-2)的电泳形态不同于原种,且分别与千粒重和籽粒长宽比及株高和抽穗期的变异相关。

PRELIMINARY STUDY ON MUTAGENIC EFFECTS OF ACCELERATED ARGON ION IRRADIATION IN RICE

Dry seeds of a.indie a rice variety were irradiated by argon ion beam (E=400MeV/u,LET=117keV/μm)with various doses.The injurious effects in M1 generation were observed,and the dose-seedling survival curve as well as dose-fertility curve were established,which showed that D50-s and D50-f were 93.7 Gy and 67.0 Gy respectively.The mutants with variation in quantitative or morphological characters were sought in M2 generation and the results indicated that treatments at dose from 60 Gy to 120 Gy were effective in inducing mutation,especially for mutation of semidwarf,ear-lyness,and large grain,but the highest mutation frequency was observed at 90 Gy treatment.Also,more than one mutated-characters were frequently found in one single plant.An analysis of nine isozymes were conducted to d'etect linkage between isozyme loci and loci controlling mutated characters,and the alteration of electrophoretic banding patterns was observed in most mutants surveyed,when compared with original variety,at one or two enzymes of PGD-1 and PGI-2,which related to mutation on characters of grain shape and plant height respectively.