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| 甜瓜组织培养再生植株中的四倍体变异 | |
| 引用本文： | 马国斌,王鸣,郑学勤. 甜瓜组织培养再生植株中的四倍体变异[J]. 园艺学报, 1999, 26(2): 128-130 |
| 作者姓名： | 马国斌 王鸣 郑学勤 |
| 作者单位： | 1. 新疆农业大学西瓜甜瓜研究所,乌鲁木齐830052 2. 西北农业大学园艺系,陕西杨凌712100 3. 热带作物生物技术国家重点实验室,海口571100 |
| 基金项目： | 热带作物生物技术国家重点实验室资助项目 |
| 摘 要： | 甜瓜未成熟子叶，幼苗子叶和真叶离体培养的再生植株中存在着明显的四倍体变异，比率分别为３０％，１６．２％和４２．９％，四倍体植株在花冠，气孔，花粉，种子和果实的形态和大小方面均与二倍体植株表现明显的差异。再生植株叶片气孔大小和气孔保卫细胞内叶绿体数目可以作为从再生植株中早期鉴别四倍体植株的可靠指标。组织培养可以成为获得四倍体甜瓜的有效途径。 |
| 关 键 词： | 甜瓜 组织培养 四倍体 再生植株 |
| 修稿时间： | 1998-04-20 |
Generating Tetraploid Plants from Tissue Culture of Melon |
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| Ma Guobin,Wan Ming,Zheng Xueqin. Generating Tetraploid Plants from Tissue Culture of Melon[J]. Acta Horticulturae Sinica, 1999, 26(2): 128-130 | |
| Authors: | Ma Guobin Wan Ming Zheng Xueqin |
| Abstract: | Variation of tetraploidy was observed among the regenerants from culture of immature cotyledons,cotyledons and leaves of melon seedlings.Tetraploid plants exhibited sighificant difference in morphology of flowers,pollens,stomates,shape and size of seeds and fruits comparted with the diploidy.The stoma size and chloroplast number in guard cells could be regarded as the reliable indicators of ploidy levels to identify seedlings in the early stage.Tissue culture was a new and efficient way to obtain tetraploid melon. |
| Keywords: | Melon(Cucumis melo L.) Tissue culture Tetraploidy |
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