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TERF1基因增强糖橙抗病性的初步研究
引用本文:罗赛男,邓子牛,易图永,黄荣峰,曾柏全.TERF1基因增强糖橙抗病性的初步研究[J].中南林业科技大学学报,2008,28(5).
作者姓名:罗赛男  邓子牛  易图永  黄荣峰  曾柏全
作者单位:湖南农业大学园艺园林学院,国家柑橘改良中心长沙分中心,湖南农业大学生物安全技术学院,湖南省农业科学院园艺研究所,中国农业科学院生物技术研究所,中南林业科技大学生命科学与技术学院
基金项目:国家柑橘转基因专项基金  
摘    要:将获得转TERF1基因糖橙的春梢叶片进行柑桔溃疡病和炭疽病的接种.初步试验表明,TERF1基因增强了糖橙的广谱抗病性.在接种溃疡病后,转基因植株的平均发病率下降了50%左右,且发病的时间比冰糖橙和对照推迟了7 d,其中3号株系的抗病性最强,叶片无病症.柑橘叶片接种炭疽病后,转基因植株的叶片与对照相比基本上表现出针孔处病斑减小,延迟发病等现象.其中3号和11号转TERF1基因糖橙叶片上根本观察不到炭疽病的典型病斑,且叶片也无褪绿.

关 键 词:生物技术  果树育种  糖橙  TERF1基因  柑橘溃疡病  柑橘炭疽病

Preliminary Results of Succari's Disease Resistance Enhanced with TERF1 Gene
LUO Sai-nan,DENG Zi-niu,ZHONG Xiao-hong,YI Tu-yong,HUANG Rong-feng,ZENG Bai-quan.Preliminary Results of Succari's Disease Resistance Enhanced with TERF1 Gene[J].Journal of Central South Forestry University,2008,28(5).
Authors:LUO Sai-nan  DENG Zi-niu  ZHONG Xiao-hong  YI Tu-yong  HUANG Rong-feng  ZENG Bai-quan
Abstract:An experiment was conducted by inoculating citrus bacterial canker and citrus anthracnose to the leaves of transgenic Succari.Results show that TERF1 gene can enhance the transgenic plants with their broad-spectrum disease resistance; that after an inoculation with citrus bacterial canker,their incidence rate is reduced by 50%;that compared to Bingtang Orange and CK,transgenic Succari also delays the expression of disease symptoms in 7 days and the inoculated plant No.3 has displayed no symptoms at all;that after an inoculation with citrus anthracnose,the contrast plants display typical symptoms in 7 days but the transgenic plants show a size reduction of diseased spots and a delay of diseased time;and that the inoculated plants No.3 and No.11 even show evident resistance to citrus anthracnose.
Keywords:biotechnology  fruit breeding  Succari  TERF1 gene  citrus bacterial canker  citrus anthracnose
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