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| 视频扫描测定油菜饼粕自由基清除活力方法的研究 | |
| 引用本文： | 李培武,张文,杨湄,丁小霞,Anu Hopia,Jari Summanen,Teijo Yrjonen,Heikki Vuorela.视频扫描测定油菜饼粕自由基清除活力方法的研究[J].中国油料作物学报,2001,23(1):60-65. |
| 作者姓名： | 李培武 张文 杨湄 丁小霞 Anu Hopia Jari Summanen Teijo Yrjonen Heikki Vuorela |
| 作者单位： | 1. 中国农业科学院油料作物研究所，湖北 武汉430062 2. 农业部油料及制品质量监督检验测试中心，湖北 武汉430062 3. 赫尔辛基大学应用化学与微生物学系，芬兰 赫尔辛基F1N-00014 4. 赫尔辛基大学药学系，芬兰 赫尔辛基F1N-00014 |
| 基金项目： | 国家自然科学基金资助项目(39770452) |
| 摘 要： | 用视频扫描平板色谱和稳定自由基1，1－二苯基－2－间三硝苯基偕腙肼（DPPH）检测油菜饼粕自由基清除活力的方法，研究抗坏血酸，维生素E，酚酸和类黄酮等17种常见酚类化合物对自由基的清除活力，发现自由基清除面积与样品量的相关系数为0．947－0．996，检测下限为40－690ng，以维生素E指数为清除活力指标，视频扫描法与常规标准比色法的测定结果具有良好的相关性，测定油菜饼粕提取液自由基清除活力发现，68－75％的化合物具有自由基清除能力，Rf值为0．41的化合物清除活力最高，占总活力的38％。 |
| 关 键 词： | 视频扫描 油菜饼粕 平板色谱 自由基 清除活力 抗氧化剂 饲料原料 食品添加剂 |
| 文章编号： | 1007-9084(2001)01-0060-06 |
Evaluation of free radical scavenging activity of rapeseed meal by video scanning technology on TLC |
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| LI Pei-wu ,ZHANG Wen ,YANG Mei ,DING Xiao-xia ,Hopia ,Jari Summanen ,Tejio Yrjoen ,Heikki Vuorela.Evaluation of free radical scavenging activity of rapeseed meal by video scanning technology on TLC[J].Chinese Journal of Oil Crop Sciences,2001,23(1):60-65. | |
| Authors: | LI Pei-wu ZHANG Wen YANG Mei DING Xiao-xia Hopia Jari Summanen Tejio Yrjoen Heikki Vuorela |
| Institution: | LI Pei-wu 1,ZHANG Wen 2,YANG Mei 2,DING Xiao-xia 2,Hopia 3,Jari Summanen 4,Tejio Yrjoen 4,Heikki Vuorela 4 |
| Abstract: | A reversed phase thin layer chromatography (TLC) method combinedwith video scanning detection for quantitative evaluation of free radical scavenging activity of antioxidative fractions from rapeseed meal by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. DPPH scavenging activity of L-ascorbic acid and 17 well-known phenolic compounds including α-tocopherol, phenolic acids and flavonoids was determined by this TLC-DPPH method. Correlation coefficients (R2) between activity and sample amount applied were 0.947～0.996 and the detection limits were 40～690ng depending on compounds. Comparison of the results based on α-tocopherol index showed a good correlation between the activities measured by TLC-DPPH and by the conventional cuvette assay . The method was used to measure free radical scavenging activity of rapeseed meal fractions. Ten free radical scavenging bands were detected which Rf values from 0.04 to 0.85. Radical scavenging activity of the one with Rf value 0.41 was highest, possessing 38% of the total scavenging activity. |
| Keywords: | Video-scan Rapeseed meal TLC-DPPH Free radical Scavenging activity Antioxidative compounds |
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