高产优质毛白杨良种组培繁育体系构建

目的毛白杨是我国北方人工林营造、景观绿化及木材加工等产业应用的主要树种。近年来，由于林业产业升级，原有的毛白杨多圃配套繁育体系不能满足高质量毛白杨良种的快速繁育需求，因此亟需构建高效的毛白杨良种繁育技术体系以保障毛白杨良种在木材生产以及城市绿化中的广泛应用。方法以高产优质毛白杨良种‘毅杨1号’‘毅杨2号’和‘毅杨3号’为研究对象，以其茎段和叶片为试验材料，利用组织培养技术构建毛白杨良种组培繁育体系。本研究系统地分析了‘毅杨 1 号’ ‘毅杨2号’和‘毅杨3号’外植体的消毒方法及植物激素对其不定芽、生根及叶片增殖诱导过程中的作用。结果（1）75%乙醇处理30 s联合2%次氯酸钠处理5 min是3个毛白杨良种外植体的最佳消毒方法。（2）不定芽诱导中0.3 mg/L 6-BA和0.1 mg/L的NAA适用于‘毅杨 1 号’和‘毅杨2号’ ‘毅杨3号’则在0.5 mg/L 6-BA和0.1 mg/L NAA的条件下增殖系数达到最大。（3）生根诱导中‘毅杨 1 号’和‘毅杨3号’的IBA最适质量浓度为0.5 mg/L，‘毅杨2号’则为0.3 mg/L。（4）叶片增殖结果显示，‘毅杨 1 号’和‘毅杨3号’的植物激素最适质量浓度为1.0 mg/L 6-BA和0.1 mg/L NAA，‘毅杨2号’为0.7 mg/L 6-BA和0.1 mg/L NAA。结论本研究构建了‘毅杨 1 号’ ‘毅杨2号’ ‘毅杨3号’的快速繁育体系，可用于毛白杨良种的快速繁殖以及遗传转化研究，并将为毛白杨良种推广应用以及进一步利用基因工程技术在分子水平对现有良种开展精准改良奠定了基础。 

Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa

ObjectivePopulus tomentosa is mainly used in artificial forest construction, landscape greening and wood processing industries in the northern China. Due to the upgrading and transformation of forestry industry, the original breeding technology of P. tomentosa cannot be suitable to the requirement of high-quality P. tomentosa in recent years. To ensure the extensive application of improved species of P. tomentosa in wood production and urban greening, it is urgent to construct an efficient breeding technology system of P. tomentosa now.MethodThree improved forest varieties, ‘Yiyang 1’, ‘Yiyang 2’and ‘Yiyang 3’, were used for rapid breeding system construction using tissue culture technology. This study systematically analyzed the effects of disinfection methods and plant hormones on the acquisition of sterile materials, adventitious buds, rooting and leaf proliferation induction.Result(1) The best sterilized method for the three improved poplar varieties was to treat them with 75% ethanol for 30 seconds and 2% sodium hypochlorite for 5 minutes. (2) In adventitious bud induction, 0.3 mg/L 6-BA and 0.1 mg/L NAA were applicable to ‘Yiyang 1’ and ‘Yiyang 2’, while the proliferation coefficient of ‘Yiyang 3’ reached the maximum under the conditions of 0.5 mg/L 6-BA and 0.1 mg/L NAA. (3) In rooting induction, the optimal concentration of IBA of ‘Yiyang 1’and ‘Yiyang 3’ was 0.5 mg/L, and that of ‘Yiyang 2’ was 0.3 mg/L. (4) The results of leaf proliferation showed that the optimal concentrations of plant hormones were 1.0 mg/L 6-BA and 0.1 mg/LNAA in ‘Yiyang 1’ and ‘Yiyang 3’, and 0.7 mg/L 6-BA and 0.1 mg/L NAA in ‘Yiyang 2’.ConclusionThe rapid breeding system of ‘Yiyang 1’, ‘Yiyang 2’ and ‘Yiyang 3’ has been preliminarily constructed in this study, which is helpful to promote rapid propagation and genetic transformation research of these improved varieties of P. tomentosa, and lays technical foundation for the directional improvement of improved varieties of P. tomentosa at molecular level.