Study on the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes of mouse |
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Authors: | WANG Xiang ZENG Qiu-tang ZHU Wu-qiang CAO Lin-sheng |
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Institution: | Institute of Cardiovascular Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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Abstract: | AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D3 line(ES-D3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC. |
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Keywords: | Embryo Stem cells Myocardium Electrophysiology Mice |
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