Role of leukotriene D4 in promoting proliferation of cultured human airway smooth muscle cells |
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Authors: | LUO Ya-ling WEN Jin-xu XU Jian ZHU Jian-jun |
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Institution: | Department of Respiratory, Nan Fang Hospital, Guangzhou 510515, China |
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Abstract: | AIM: To investigate whether leukotriene D4(LTD4) would stimulates proliferation of cultured human airway smooth muscle (ASMC). METHOD: Human ASMC were isolated and subcultured, varying concentration of LTD4 were added to the media. Cell counts were obtained, -thymidine(3H]-TdR) incorporation and inositol 1, 4, 5-trisphosphate (IP3) accumulation were measured. RESULTS: LTD4(0.1nmol·L-1~10 nmol·L-1) increased cell number and also increased incorporation of3H]-TdR and accumulation of IP3 in a concentration dependent manner(P<0.01). The latter response was blocked by phospholipase C inhibition with neomycin (1 μmoL·L-1(P<0.01). However, neomycin had no effect on the promitogenic action of LTD4. CONCLUSION: LTD4 stimulates proliferation of cultured human ASMC and may play a role in airway remodeling of asthma. |
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Keywords: | Muscle smooth Leukotrienes Hyperplasia Asthma |
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