| 粉红聚端孢体外产毒条件的研究 |
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| 引用本文： | 李金花,柴兆祥,高彩菊.粉红聚端孢体外产毒条件的研究[J].甘肃农业大学学报,2004,39(6):675-678. |
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| 作者姓名： | 李金花 柴兆祥 高彩菊 |
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| 作者单位： | 1. 甘肃农业大学植物保护系,甘肃,兰州,730070
2. 甘肃省秦安县西川中学,甘肃,秦安,741600 |
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| 基金项目： | 甘肃省科技厅项目(甘科计[2001]21 号) |
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| 摘 要： | 采用白兰瓜种子发芽抑制试验测定了不同培养条件下粉红聚端孢(Trichotheciumroseum)培养滤液的毒性,结果表明:PSC和PSC+5%白兰瓜种子汁这两种培养基有利于该菌产生毒素,Richard产毒较少;该菌最佳产毒温度为20~25;以pH7.0最有利于产毒;振荡培养比静置培养利于产毒;该菌在连续黑暗与连续光照条件下均可产毒,连续黑暗更适宜于产毒;在设置的培养时间内,随培养时间的延长,培养滤液对种子的抑制率升高。
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| 关 键 词： | 粉红聚端孢 产毒条件 |
| 文章编号： | 1003-4315(2004)06-0675-04 |
Production condition on toxin of Trichothecium roseum in vitro |
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| LI Jin-hua,CHAI Zhao-xiang,GAO Cai-ju.Production condition on toxin of Trichothecium roseum in vitro[J].Journal of Gansu Agricultural University,2004,39(6):675-678. |
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| Authors: | LI Jin-hua CHAI Zhao-xiang GAO Cai-ju |
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| Institution: | LI Jin-hua1,CHAI Zhao-xiang1,GAO Cai-ju2 |
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| Abstract: | The toxicity on culture liquid of T. roseum was determined by the method of inhibiting germination rate of muskmelon seeds. The results showed that 2 kinds of medium (PSC and PSC+5 % seed juices) were suitable for toxin production. Toxin produced little in Richard medium. The optimum conditions were 20~25 and pH 7.0. The conditions of quivering culture and darkness showed more benefit than that of quite culture and continuous light. Germination inhibiting rate of the seeds increased with prolonged culturing time during incubation. |
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| Keywords: | Trichothecium roseum toxin production condition |
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