广西巴马小型猪脂联素受体1和受体2 cDNA的克隆及序列分析

该试验根据GenBank中普通猪脂联素受体（AdipoR）mRNA序列，利用RT—PCR法对广西巴马小型猪AdipoR基因进行克隆，与NCBI中发表的猪AdipoR序列进行同源性比较，分析广西巴马小型猪AdipoR的cDNA及氨基酸序列结构特点，为进一步从分子水平上对广西巴马小型猪的生长发育提供理论依据，也为日后构建真核表达载体，研究AdipoR基因的生物功能奠定基础。

Cloning and Sequence Analysis of Adiponectin Receptor 1 and Receptor 2 cDNA from Guangxi Bama Mini-pig

Objective] To clone and analyze the sequence of Adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) cDNA of Guangxi Bama mini-pig. Method] The Adiponectin receptors cDNAs were amplified by RT-PCR using skeletal muscle total RNA as template and then ligated into pMD18-T vector after purification. The recombinant pMD18-T vector was transformed into the E.coli DH5α for identification and sequencing. And the results were compared with the cDNA sequence from other species. Result] The fragments, 1 128 bp and 1 161 bp in size, were amplified by RT-PCR and respectively consistent with the coding sequence of AdipoR1 gene and AdipoR2 gene. The homology analysis showed that the sequences of AdipoR1 gene and AdipoR2 gene were respectively 99.8% and 99.7% homologous to the sequence of domestic pig reported in GenBank with one base and three base missense mutations correspondingly. Conclusion] The AdipoR1 gene and AdipoR2 gene were successfully amplified from Guangxi Bama mini-pig, laying the foundation for the further study of the biological function of AdipoR genes and the design of novel drugs with AdipoR as target.