| 百合花发育相关MADS box基因的克隆 |
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| 引用本文： | 张云,刘青林,欧阳青,蔡文启.百合花发育相关MADS box基因的克隆[J].园艺学报,2004,31(3):332-336. |
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| 作者姓名： | 张云 刘青林 欧阳青 蔡文启 |
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| 作者单位： | (中国农业大学观赏园艺与园林系，北京100094；中国科学院微生物研究所，北京100080) |
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| 基金项目： | 北京市自然科学基金资助项目 ( 60 42 0 15 ) |
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| 摘 要： | 利用RT-PCR的方法，从百合中克隆得到了3个接近全长的花发育相关MADS box基因片段 LfMADS1-3。其中LfMADS1和LfMADS3以往未见报道，分别与多种作物的AG、SEP同源基因具有较高的同源性；LfMADS2与已发表的LMADS2对应区段的氨基酸同源性高达98.99%，推测可能是同一个基因。
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| 关 键 词： | 百合 花发育 MADSbox基因 克隆 |
| 文章编号： | 0513-353X(2004)03-0332-05 |
| 收稿时间： | 2003-06-23 |
Cloning of Flower Development Associated MADS box Gene Fragments in Lilium |
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| Zhang Yun ,Liu Qinglin ,Ouyang Qing ,and Cai Wenqi.Cloning of Flower Development Associated MADS box Gene Fragments in Lilium[J].Acta Horticulturae Sinica,2004,31(3):332-336. |
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| Authors: | Zhang Yun Liu Qinglin Ouyang Qing and Cai Wenqi |
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| Institution: | (Department of Ornamental Horticulture and Landscape Archhecture，China Agricultural University，Beijing 100094，China; Institute of Microbiology, Chinese Academy of Sciences，Beijing 100080，China) |
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| Abstract: | LfMADS 1-3,three MADS box cDNA fragments associated with flower development were cloned from Lilium using a RT PCR based cloning method. LfMADS 1 and LfMADS 3 are new MADS box genes in lilies,which have not been reported before.They showed high sequence homology with AG and SEP homologs,respectively.The deduced amino acid sequence of LfMADS 2 shared 98 99% identity with the reported LMADS 2, which suggested that they might be the same gene. |
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| Keywords: | Lilium Flower development MADS box gene Cloning |
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