草梅离体试管苗光合作用的研究

无病毒草莓丰香（Ｆｒａｇａｒｉａ　ａｎａｎａｓｓａ Ｄｕｃｈ．ｃｖ．Ｆｅｎｇｘｉａｎｇ）离体试管苗具有光合能力，但其光合作用受培养基中的蔗糖浓度、培养光强及培养容器内ＣＯ２浓度的影响。在无蔗糖或含有较低浓度蔗糖的培养基上和较高的培养光强下生长的试管苗，其净光合速率（Ｐｎ）、光饱和点和表观量子效率（ＡＱＹ）较高，光补偿点较低。生长有小苗的培养容器内的ＣＯ２浓度只有７０－１２０μｌ·ｌ－１，远远低于大气的ＣＯ２浓度。随着向培养容器中通入ＣＯ２浓度的增加，试管苗的Ｐｎ增加。培养６周试管苗的Ｐｎ高于培养３周的试管苗，培养容器内的ＣＯ２浓度６周培养的低于３周。

Studies on Photosynthesis of Strawberry Plantlets in vitro

In-vitro-cultured virus-free strawberry (Fragaria ananassa Duch. cv. Fengxiang) plantlets have nored Photosynthetic capacity, however, their photosynthesis were affected by levels of sucrose in culture medium, illumination and carbon dioxide concentrations in culture vessels. The net photosynthetic rate(Pn), light saturation points and apparent quantum yield(AQY) were increased in sucrose-free or lower sucrose concentrantion and higher light intensity for growth of planilets, but light compensation points was reduced. The carbon dioxide concentrations in culture vessels were only 70 to 120μl·l-1,which were far lower than that in the atmospher. When the carbon dioxide increased in the vessels, the Pn was increased. The Pn in those plantlets clutured after 6 weeks was higher than that cultured for 3 weeks, while the carbon dioxide concentrations was on the contrary.