京海黄鸡IGFBP-3基因外显子1和内含子1部分序列多态性及其与生长繁殖性状的相关性

以京海黄鸡母鸡为材料，采用PCR-SSCP技术对IGFBP-3基因外显子1及内含子1部分序列多态位点进行研究，并计算基因型频率、基因频率、卡方值和部分遗传多态性指标。结果表明，在外显子1上没有检测到多态位点，在内含子1上检测到1个多态位点，该位点为中度多态，在160 bp处发生T到G的突变，导致BB、AB型的56日龄体重显著大于AA型(P＜0.05)，AA型的11月产蛋数显著大于BB、AB型(P＜0.05)。由此初步推断，内含子1对产蛋及生长发育很可能有一定的促进作用，A为产蛋的优势基因，B为体重的优势基因。

Genetic Diversity of IGFBP-3 Gene Exon 1 and Partial Sequence of Intron 1 and their Association with Growth and Reproductive

To Jinghai yellow chicken hens as materialusing PCR-SSCP technique to study IGFBP-3 gene exon 1 and partial sequence of intron 1 polymorphic site and calculate the genotype, gene frequency, chi-square value and some indicators of genetic polymorphism. The results of exon 1 was not detected polymorphic site, in intron 1 of the partial sequence detected in more than one polymorphic site, the locus polymorphism as a moderate, in the first 160 bp took place T to G mutation, leading to BB and AB type 56 days of age, body weight was significantly greater than AA type (P<0.05), AA type egg in November was significantly greater than the number of BB and AB type (P<0.05). From this initial inference of intron 1 on the egg-laying and growth was likely to have a certain role in promoting, A gene for the advantage of egg-laying, B gene for the advantage of weight.