RP-HPLC法测定博落回总碱中白屈菜红碱含量的方法研究

建立博落回总碱中白屈菜红碱的RP—HPLC含量测定方法。采用日本资生堂ODS—MG C18液相色谱柱（4．6mm×150mm，5μm）；以乙腈-0．1％磷酸溶液（45：55）（每100mL加十二烷基磺酸钠0．1g）为流动相；检测波长270nm；流速为1．0mL／min。结果显示，白屈菜红碱峰形对称，拖尾因子低于1．5；在0．1μg～0．9μg范围内峰面积与浓度呈良好的线性关系（r=0．9999，n=5）；在80％、100％、120％三种添加水平下的平均回收率分别为97．4％（RSD：0．7％，n=3）、98．6％（RSD=0．1％，n=3）、99．5％（RSD=0．8％，n=3）。利用该法测定博落回总碱中的白屈菜红碱，结果准确度高、精密度好，可作为博落回总碱的质量控制方法。

Research on the Method for Determination of Chelerythrine in Total Alkaloid of Macleaya Cordata by RP-HPLC

To develop an RP - HPLC method for the determination of Chelerythrine in total alkaloid of macleaya cordata. The Shiseido ODS - MG C18 column （4.6 mm × 150 mm, 5μm） was used with acetonitrile -0.1% phosphoric acid solution （45： 55） （0.1 g sodium dodecyl sulfonate in 100 mL solution） as mobile phase. The detection wavelength was 270 nm. The flow rate was 1.0 mL/min. Results show that a show that a symmetrical peak of chelerythrine was obtained and the tailing factor was less than 1.5. The linearity range was 0.1μg - 0.9 μg （r =0.999 9,n =5） and under 80% ,100% ,120% level of accession, the average recoveries of chelerythrine were respectively97.4% （RSD=0.7%,n=3）, 98.6% （RSD =0.1% ,n =3） , 99.5% （RSD=0.8%,n=3）. The method is sensitive and reliable and can be used as a quality control method for the chelerythrine in total alkaloid of macleaya cordata.