| 三种ELISA方法检测苹果褪绿叶斑病毒和苹果茎沟病毒的比较 |
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| 引用本文： | 吴雅琴,陈霜莹,王文慧,王小凤.三种ELISA方法检测苹果褪绿叶斑病毒和苹果茎沟病毒的比较[J].植物保护学报,1998,25(3):245-248. |
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| 作者姓名： | 吴雅琴 陈霜莹 王文慧 王小凤 |
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| 作者单位： | [1]河北农林科学院昌黎果树研究所 [2]中国科学院微生物研究所 |
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| 摘 要： | 苹果褪绿叶斑病毒（ＡＣＬＳＶ）和苹果茎沟病毒（ＡＳＧＶ）是感染苹果和其它一些果树的重要病毒。作者应用ＰＡＳ－ＥＬＩＳＡ法成功地检测了苹果组培苗中的这两种病毒。为了简化操作步骤，试验了ＤＡＳ－ＥＬＩＳＡ和改良ＤＡＳ－ＥＬＩＳＡ法，并与ＰＡＳ－ＥＬＩＳＡ的检测结果比较。试验结果表明，ＤＡＳ－ＥＬＩＳＡ能检测出ＡＳＧＶ，却不能检测出ＡＣＬＳＶ，与此同时，这两种病毒均可用改良ＤＡＳ－ＥＬＩＳＡ检测。ＤＡＳ
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| 关 键 词： | 苹果 褪绿叶斑病毒 茎沟病毒 ELISA |
COMPARISON OF THREE ELISA METHOD FOR DETECTION OF APPLE CHLORITIC LEAFSPOT VIRUS AND APPLE STEM GROOVING VIRUS |
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| Wu Yaqin,Chen Shuangying,Wang Wenhui and Wang Xiaofeng.COMPARISON OF THREE ELISA METHOD FOR DETECTION OF APPLE CHLORITIC LEAFSPOT VIRUS AND APPLE STEM GROOVING VIRUS[J].Acta Phytophylacica Sinica,1998,25(3):245-248. |
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| Authors: | Wu Yaqin Chen Shuangying Wang Wenhui and Wang Xiaofeng |
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| Institution: | Institute of Fruit Trees, Hebei Academy of Agriculture and Forestry Sciences, Changli,Institute of Fruit Trees, Hebei Academy of Agriculture and Forestry Sciences, Changli,Institute of Microbiology, Chinese Academy of Sciences, Beijing and Institute of Microbiology, Chinese Academy of Sciences, Beijing |
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| Abstract: | PAS-ELISA method was successfully used to detect apple chlorotic leafspot virus (ACLSV) and apple stem grooving virus (ASGV), which are important virus diseases in fruit trees, in tissue culture apple tree seedlings. DAS-ELISA and modified DAS-ELISA techniques were compared with PAS-ELISA to simplify the operation procedures. The result showed that DAS-ELISA could detect ASGV but ACLSV. Modified DAS-ELISA could detect both viruses. Both DAS-ELISA and modified DAS-ELISA techniques could shorten the detection time of ACLSV and ASGV. The techniques, however, have higher strain specificity. The false negatives can be caused therefore. |
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| Keywords: | ACLSV ASGV ELISA comparison |
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