注射白斑综合征病毒对克氏原螯虾酚氧化酶活力的影响

将白斑综合征病毒(white spot syndrome virus,WSSV)、嗜水气单胞菌(Aeromonas hydrophila,Ah)、大肠杆菌(Escherichia coli)(DH5α)用注射法接种克氏原螯虾(Procambarus clarkii),在0~72 h之间定时检测克氏原螯虾血细胞和肝胰脏中酚氧化酶(Phenoloxidase,PO)活力变化。结果显示:(1)0.1 mg/m L和1 mg/m L胰蛋白酶处理样品后,样品间差异不显著。(2)加胰蛋白酶处理与未加胰蛋白酶相比,供试克氏原螯虾PO活力均升高。(3)未加胰蛋白酶与加胰蛋白酶表现出相似的特征,WSSV和Ah注射组与对照组相比均表现为,12~48 h PO活力显著高于对照组,并且在48 h达到最大值,72 h时基本恢复正常;注射DH5α组与对照组相比没有显著性变化。可见感染WSSV后,克氏原螯虾体内酚氧化酶活力发生了变化,由此推测,PO参与了螯虾体内抵御病毒的免疫反应。

Phenoloxidase activity of Procambarus clarkii infected by white spot syndrome virus

Healthy red swamp crayfish, Procambarus clarkii were infected by white spot syndrome virus(WSSV), Aero-monas hydrophila, and Escherichia coli( DH5ɑ) respectively. Phenoloxidase activity in crayfish hemocytes and hepatopan-creas were examined from 0~72 h post infection at regular time. The results showed as follows:(1) The difference of PO activity was not significant after treated with trypsin at 0. 1 mg/mL and 1 mg/mL. (2) Compared with none trypsin, PO ac-tivity of P. clarkii was increased via the trypsin pathway. (3) Whether added trypsin or not, it had the similar characteris-tics, that the PO activity in WSSV and Ah injection groups were significantly higher than that of the control group after 12~48 h and reached to the top level at 48 h respectively. It finally returned to original level after 72 h;The changes of PO activity were not significant between DH5ɑ experimental and control groups. PO activity of P. clarkii was changed after WSSV infection. We can speculated that PO involved in antiviral immune response of P. clarkii in vivo.