MSTN及FGF5基因编辑不改变绒山羊母羊同期发情能力

基因编辑动物是研究动物生产性状及基因功能的良好载体,目前关于各类基因编辑动物的研究广泛开展。然而,基因编辑动物繁殖性能方面是否与野生型个体具有明显差异则尚未见报道。本研究拟通过比较野生型绒山羊与MSTN、FGF5基因编辑绒山羊对同期发情处理的响应差异,确定其与野生型绒山羊同期发情效率的差异。以野生型和MSTN、FGF5基因编辑陕北白绒山羊为研究对象,在繁殖季节采用同种处理方式(CIDR + PG)对试验羊只进行处理,研究两类羊只在繁殖季节同期发情效率的差异。结果表明,野生型绒处理后同期发情率为100%,基因编辑绒山羊处理后同期发情率也为100%,两组无差异；比较MSTN和FGF5基因编辑的羊只在处理后同期发情效率的差异,发现两组无差异,与野生型绒山羊相比同样无差异。本研究结果证实了MSTN及FGF5基因编辑后不影响母羊的繁殖性能,为基因编辑动物的推广应用提供了试验基础。

MSTN and FGF5 Gene Editing Does Not Affect Estrus Synchronization of Cashmere Goats

In this study, we aim to compare the responses of wild-type cashmere goats withMSTN- orFGF5-editing cashmere goats on the hormones for estrus synchronization, then find out the difference of estrus synchronization efficiency between the wild-type and the gene-editing cashmere goats. The goats were treated with the same method (CIDR + PG) in the breeding season. The results showed that the estrus synchronization rate of the wild-type cashmere goats was 100 % after the treatment, and that of the gene-editing goats was also 100% . Moreover, the MSTN- and FGF5- editing goats showed no significant difference in the estrus synchronization efficiency, and also showed no significant difference in that with the wild-type goats. The results confirmed thatMSTN andFGF5 gene editing did not affect the reproductive performance of cashmere goats. The results provide an experimental basis for the application of gene editing animals.