| 香稻PRO基因的克隆及表达载体的构建 |
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| 引用本文： | 田华,徐春霞,段美洋,黎国喜,唐湘如.香稻PRO基因的克隆及表达载体的构建[J].华北农学报,2009,24(3). |
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| 作者姓名： | 田华 徐春霞 段美洋 黎国喜 唐湘如 |
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| 作者单位： | 1. 华南农业大学农学院,广东广州,510642
2. 云南省农业科学院粮食作物研究所,云南昆明,650205 |
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| 基金项目： | 中国博士后科学基金，国家自然科学基金项目，高等学校博士学科点专项科研基金，广东省自然科学基金项目，广东省农业攻关重点专项 |
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| 摘 要： | 利用逆转录一聚合酶链式反应(RT-PCR)得到在水稻中特异性表达的脯氨酸氧化酶(PRO)基因.DNA序列分析表明,所获得水稻的PRO cDNA的最大开放阅读框序列全长为1 428 bp,可编码476个氨基酸.该序列与NCBI网站上已发表的PRO基因100%相似.为了能进一步验证所克隆的序列是我们所需的目的基因,成功构建了PRO基因超表达载体和PRO基因干涉载体,便于导入水稻中进行基因的功能鉴定.
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| 关 键 词： | 香稻 脯氨酸氧化酶 基因 克隆 表达载体 |
Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector |
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| TIAN Hua,XU Chun-xia,DUAN Mei-yang,LI Guo-xi,TANG Xiang-ru.Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector[J].Acta Agriculturae Boreali-Sinica,2009,24(3). |
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| Authors: | TIAN Hua XU Chun-xia DUAN Mei-yang LI Guo-xi TANG Xiang-ru |
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| Institution: | 1.College of Agronomy;South China Agricultural University;Guangzhou 510642;China;2.Institute of Food Crops;Yunnan Academy of Agriculture Sciences;Kunming 650205;China |
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| Abstract: | By RT-PCR method the cDNA of PRO gene was obtained.Analysis of DNA sequence showed that the largest open reading frame sequence of PRO cDNA was 1 428 bp,which could encode 476.amino acids.The sequence with the NCBI web site has been published by the PRO gene 100% similarity.In order to further verify the sequence by which we cloned the gene required for the successful construction of a over expression PRO gene vector and interference expression PRO gene vector,to facilitate the import of rice carried out to... |
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| Keywords: | Aromatic rice Proline oxidase Gene Cloning Expression vector |
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