信阳水牛PRL基因单核苷酸多态性与生长性状的相关性

以50头信阳水牛为材料,以GenBank公布的牛PRL基因序列(NC_007324)作为参照序列,设计PCR引物,对PRL基因的第2、4、5外显子进行变异检测,并将其多态性与部分生长性状进行相关分析.结果表明:试验牛群体中,每一外显子的PCR扩增产物经SSCP分析均出现不同带型；试验牛PRL基因第2、4、5外显子各存在...

Associations between single nucleotide polymorphisms in PRL gene and growth traits in Xinyang buffalo

Blood samples from fifty xinyang buffalos were extracted for genomic DNA and the primers for PRL gene (NC007324) were designed to detect the polymorphisms in the second，fourth and fifth exons of this gene by PCRSSCP and DNA sequencing， then the correlations between the polymorphisms and the growth traits of the buffalos were analyzed. The PCRSSCP and DNA sequencing showed that in the tested buffalos each exon had shifted SSCP band and there was one SNP in each exon (2974G>A，7581G>A，8558 G>). The correlation analysis showed that the hucklebone widths in the buffalos genotyped AA were significantly (P<0.05) greater than those in the buffalos genotyped BB based on the second exon polymorphisms.