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| 农杆菌介导的PEPCase基因导入小麦的初步研究 | |
| 引用本文： | 贾栋,张彬,马建军. 农杆菌介导的PEPCase基因导入小麦的初步研究[J]. 山西农业大学学报(自然科学版), 2009, 29(4): 323-326 |
| 作者姓名： | 贾栋 张彬 马建军 |
| 作者单位： | 1. 山西农业大学,农学院,山西,太谷,030801 2. 山西农业大学,园艺学院,山西,太谷,030801 3. 朔州市平鲁区职业中学,山西,朔州,036800 |
| 基金项目： | 山西省科技攻关项目，山西农业大学科技创新基金 |
| 摘 要： | 利用根癌农杆菌遗传转化系统,以普通小麦扬麦158为材料,将玉米泛生素基因启动子(Ubi)驱动下的磷酸烯醇式丙酮酸羧化酶(PEPCase)基因,以及潮霉素磷酸转移酶基因(hpt)导入预培养10~12 d小麦胚性愈伤组织中。经过在含100~150 mg.L-1潮霉素(hyg)的筛选培养基上连续筛选,获得了hyg抗性植株。经GUS组织化学染色检测和PCR鉴定,其中的9棵含有Ecppc基因,PCR阳性植株比例为3.03%。初步鉴定已成功地建立了小麦遗传转化系统,为进一步探讨PEPCase基因在植物中的转化和表达奠定了基础。 |
| 关 键 词： | 小麦 磷酸烯醇式丙酮酸羧化酶(PEPCase) 农杆菌介导法 转基因植株 |
Agrobacterium Tumefaciens Mediated PEPCase Gene Transformation to Wheat |
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| JIA Dong,ZHANG Bin,MA Jian-jun. Agrobacterium Tumefaciens Mediated PEPCase Gene Transformation to Wheat[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2009, 29(4): 323-326 | |
| Authors: | JIA Dong ZHANG Bin MA Jian-jun |
| Abstract: | PEPCase gene drived by ubiquiten promoter and hygromycin phosphotransferase gene hpt was transformed to yangmai158 mediated by Agrobacterium tumefaciens.Regeneration plant was inducted from 10~12 day wheat immature embryos.Hygromycin resistant(hyg) plantlets were obtained by 2~3 cycles screening in selective medium containing 100~150 mg·L-1 hygomycin.By means of PCR amplification and GUS histochemical analysis of hyg-resistant plants showed that the target gene had been integrated into wheat accompany with hpt gene.The frequency of transformation was 3.03%. |
| Keywords: | Wheat Phosphoenolpyruvate carboxylase gene Agrobacterium infection Transgenic plant |
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