Enzymatic epoxidation of Sapindus mukorossi seed oil by perstearic acid optimized using response surface methodology |
| |
Authors: | Shangde Sun Xiaoqiao KeLonglong Cui Guolong YangYanlan Bi Fanfan SongXiadi Xu |
| |
Institution: | Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, 140 Songshan South Road, Zhengzhou 450052, Henan Province, PR China |
| |
Abstract: | As a novel renewable resource, Sapindus mukorossi seed oil (SMSO) with an iodine value of 84.86 g/100 g, and containing 51.0 ± 0.9% oleic acid (18:1), 6.6 ± 0.6% linoleic acid (18:2), 1.1 ± 0.3% linolenic acid (18:3), and 23.1 ± 0.9% eicosanoic acid (20:1), was epoxidized using hydrogen peroxide as oxygen donor and stearic acid as active oxygen carrier in the presence of immobilized Candida antarctica lipase B. The effect of the amount of stearic acid on the enzymatic epoxidation was investigated. Response surface methodology (RSM) was used to study and optimize the effects of variables (reaction temperature, enzyme load, mole ratio of H2O2/CC-bonds, and reaction time) on the epoxy oxygen group content (EOC) of epoxidized SMSO. Results showed that stearic acid as active oxygen carrier could enhance the enzymatic epoxidation of SMSO. The variables of reaction temperature and enzyme load were the most significant in the process. A two second-order model was satisfactorily fitted the data (R2 = 0.9723) with non-significant lack of fit. The optimum EOC of epoxidized SMSO was 4.6 ± 0.3% under the conditions of 50.0 °C, 7.0 h, 2.00% (relative to the weight of SMSO) enzyme load, and 4:1 mole ratio of H2O2/CC-bonds. |
| |
Keywords: | Epoxidation Sapindus mukorossi seed oil Immobilized Candida antarctica lipase B Stearic acid Response surface methodology |
本文献已被 ScienceDirect 等数据库收录! |
|