| 蝴蝶兰EST资源的SSR信息分析 |
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| 引用本文： | 李冬梅,吕复兵,朱根发,洪彦彬,陈小平,梁炫强,操君喜,孙映波.蝴蝶兰EST资源的SSR信息分析[J].广东农业科学,2011,38(3):117-120. |
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| 作者姓名： | 李冬梅 吕复兵 朱根发 洪彦彬 陈小平 梁炫强 操君喜 孙映波 |
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| 作者单位： | 李冬梅,吕复兵,朱根发,操君喜,孙映波,LI Dong-mei,LV Fu-bing,ZHU Gen-fa,CAO Jun-xi,SUN Ying-bo(广东省农科院花卉研究所/广东省园林花卉种质创新综合利用重点实验室,广东,广州,510640);洪彦彬,陈小平,梁炫强,HONG Yan-bin,CHEN Xiao-ping,LIANG Xuan-qiang(广东省农科院作物研究所,广东,广州,510640) |
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| 基金项目： | 广东省农业攻关项目，广东省农业科学院支撑计划项目 |
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| 摘 要： | 对5 472条蝴蝶兰无冗余EST序列进行了SSR搜索,共检索出 376条EST含有419个SSR位点,平均每7.4 kb EST序列出现1个SSR.共有60种SSR的重复基元.其中二核苷酸和三核苷酸重复基元占丰导地位,分别占SSR总数的67.1%和30.3%:AG/CT在SSR基元中出现频率最高(46.1%).AT/A...
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| 关 键 词： | 蝴蝶兰 EST SSR信息 |
Analysis of SSR information in EST resource of Phalaenopsis spp. |
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| LI Dong-mei,LV Fu-bing,ZHU Gen-fa,HONG Yan-bin,CHEN Xiao-ping,LIANG Xuan-qiang,CAO Jun-xi,SUN Ying-bo.Analysis of SSR information in EST resource of Phalaenopsis spp.[J].Guangdong Agricultural Sciences,2011,38(3):117-120. |
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| Authors: | LI Dong-mei LV Fu-bing ZHU Gen-fa HONG Yan-bin CHEN Xiao-ping LIANG Xuan-qiang CAO Jun-xi SUN Ying-bo |
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| Institution: | LI Dong-mei1,LV Fu-bing1,ZHU Gen-fa1,HONG Yan-bin2,CHEN Xiao-ping2,LIANG Xuan-qiang2,CAO Jun-xi1,SUN Ying-bo1(1.Floricultural Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou 510640,China,2.Crops Research Institute,Guangdong Academy of Agricultural Sciences,China) |
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| Abstract: | Simple sequence repeats(SSRs) were searched in 5 472 qualified and non-redundant expressed sequence tags(ESTs) of Phalaenopsis.A total of 376 ESTs of Phalaenopsis contained 419 SSRs with 60 kinds of repeat motifs and 1 SSR in 7.4 kb EST.Among them,the dinucleotide and trinucleotide repeats were dominant SSR types,accounting for 67.1% and 30.3%,respectively.AG/CT was most frequent SSR motif accounting for 46.1%,and then AT/AT accounted for 18.6%.Moreover,100 ESTs of Phalaenopsis were selected and designed 10... |
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| Keywords: | EST |
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