| 一株芽孢杆菌16S rRNA的基因序列测定和系统进化分析 |
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| 引用本文： | 李瑞芳,赵玉峰,薛雯雯,田泱源,张长付.一株芽孢杆菌16S rRNA的基因序列测定和系统进化分析[J].广东农业科学,2011,38(3):121-122,125. |
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| 作者姓名： | 李瑞芳 赵玉峰 薛雯雯 田泱源 张长付 |
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| 作者单位： | 河南工业大学生物工程学院,河南,郑州,450001 |
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| 基金项目： | 河南省教育厅自然科学计划项目，郑州市科技攻关项目 |
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| 摘 要： | 从河南黄河稻区土壤中分离出一株具有较强稻瘟病菌拮抗活性的芽孢杆菌菌株BS501,经过形态观察、生理生化特性检测和电镜技术,确定其为芽孢杆菌属细菌,为进一步确定其分类学地位,扩增了 BS501基因组的16S rDNA.并将测序结果提交GenBank数据库(登录号:FJ755787).利用BLAST软件将该序列与枯草芽孢杆菌、地衣芽孢杆菌等芽孢菌属细曲及大肠杆菌16S rDNA序列进行同源性比对.系统进化树表明,BS501与芽孢菌属细菌存进化关系上组成一个大的分支,与枯草芽孢杆菌最为接近,与大肠杆菌较远.同源性分析表明该序列与枯草芽孢杆菌PRE23同源性达99%,证实该菌株的分类学地位为枯草芽孢杆菌.
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| 关 键 词： | 芽孢杆菌 基因测序 系统进化分析 |
Sequencing and phylogenetic analysis of the partial 16S rRNA in a Bacillus strain |
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| LI Rui-fang,ZHAO Yu-feng,XUE Wen-wen,TIAN Yang-yuan,ZHANG Chang-fu.Sequencing and phylogenetic analysis of the partial 16S rRNA in a Bacillus strain[J].Guangdong Agricultural Sciences,2011,38(3):121-122,125. |
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| Authors: | LI Rui-fang ZHAO Yu-feng XUE Wen-wen TIAN Yang-yuan ZHANG Chang-fu |
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| Institution: | LI Rui-fang,ZHAO Yu-feng,XUE Wen-wen,TIAN Yang-yuan,ZHANG Chang-fu(College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China) |
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| Abstract: | A Bacillus strain BS501 which had strong antagonistic activity of M.grisea was isolated from the soil of rice field near the Yellow river in Henan.BS501 was determined to be a Bacillus bacteria through morphological observation,physiological and biochemical characterization and electronic microscopy.To further determine the taxonomic status of the strain,the 16S rDNA of BS501genome was amplified,and the sequencing result was submitted to the GenBank database(GenBank accession number is FJ755787).The homolog... |
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| Keywords: | 16S rDNA |
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