应用PAPD方法对鸡败血霉形体DNA多态性的研究

用随机扩增多态性ＤＮＡ（ＲＡＰＤ）方法，通过筛选的５个随机引物（ＯＰＨ－０２，ＯＰＨ－０５，ＯＰＨ－０７，ＯＰＨ－１３，ＯＰＧ－１６），对１４株鸡败血霉形体（ＭＧ）国际标准株和国内分离株进行了ＤＮＡ多态性研究。结果表明，５个引物共产生２１种条带，其中有３个条带分别为２个菌株所特有，扩增产物片段的长度在１５０～４５００ｂｐ之间，所有菌株均有１条共同条带，以ＯＰＨ－０５扩增产物的多态性最丰富。根据样品ＤＮＡ所获得的菌株间相似性指数表明，Ｄ９６０３与Ｄ９６０７相似指数最高，Ｓ６，Ｋ３９１３和Ｄ９６０４三者相互间相似性指数最低。提示ＲＡＰＤ方法可用于霉形体遗传标记的分析。

Analysis of DNA Polymorphism of Mycoplasma gallisepticum by RAPD

Five screened random primers (OPH02,OPH05,OPH07,OPH13,OPG16) were used to detect DNA polymorphism of 14 strains (R,S6,K3913,g1F8,F166,FMF3 and 8 field isolate strains). The results showed that total 21 bands were produced, 2 strains share 3 specific bands. Fragment lengths of the amplied products are generally from 150 bp tp 4.5 kb and all the strains share a same band. The most abundant polymorphism was brought about by OPH05 primer. The similar index between strains according to pooled DNA is the biggest both D9603 and D9607, and is the smallest among S6, K3913 and D9604. In conclusion, RAPD technique could be used as a means in analysing gene structure of Mycoplasama.