| 虎舌红叶片不定梢诱导研究 |
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| 引用本文： | 曾云英,李华锋.虎舌红叶片不定梢诱导研究[J].安徽农业科学,2009,37(17):7854-7854. |
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| 作者姓名： | 曾云英 李华锋 |
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| 作者单位： | 九江学院土建院,江西九江,332005;九江学院土建院,江西九江,332005 |
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| 基金项目： | 江西省教育厅科技项目 |
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| 摘 要： | 目的]建立虎舌红的高频再生体系，为利用转基因技术改良其品质奠定基础。方法]以虎舌红试管苗顶端叶片为试材进行组织培养和不定梢诱导试验。结果]外植体在9种培养基上均能诱导出愈伤组织，4号培养基的不定芽再生率最高，为45．80％，植株生长健壮；其次是7号培养基，其不定芽再生率为39．76％。从整体上看，不定芽的再生率都不高，最高的也未超过50．00％。综合考虑愈伤组织诱导和不定芽再生，适合虎舌红叶片不定芽再生的培养基为：1／2 MS＋TDZ 2．0mg／L＋IAA0．2～0．4mg／L＋AgNO3 3．0～5．0mg／L。选健壮苗接种于1／2MS＋IBA 0．3mg／L＋NAA 0．5mg／L培养基上，20d后生根率可达90％，炼苗后再生植株的移栽成活率达80％以上。结论]该研究成功建立了虎舌红叶片的离体再生体系。
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| 关 键 词： | 虎舌红 叶片 不定梢再生 |
Study on the Induction of Adventitious Shoots from Ardisia mamillata Leaves |
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| ZENG Yun-ying et al.Study on the Induction of Adventitious Shoots from Ardisia mamillata Leaves[J].Journal of Anhui Agricultural Sciences,2009,37(17):7854-7854. |
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| Authors: | ZENG Yun-ying |
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| Institution: | ZENG Yun-ying et al (College of Civil Engineering , Architecture,Jiujiang University,Jiujiang,Jiangxi 332005) |
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| Abstract: | Objective] The aim of the study was to establish the high-frequency regeneration system of Ardisia mamillata and lay a basis for improving its quality by transgenic technology.Method] With the apical leaves of tube A.mamillata seedlings as tested materials,its tissue culture and induction experiment of adventitious shoots were performed.Result] The calli could be induced from all the explants growing on the 9 media.On Medium 4,the regeneration rate of adventitious buds was highest,being 45.80% and the pl... |
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| Keywords: | Ardisia mamillata Leaves Regeneration of adventitious shoots |
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