南京地区豇豆蚜传花叶病毒的鉴定

 1982年5月,从南京郊区豇豆花叶病植株上分离到1株病毒分离物C-1,接种试验的结果证明,它可以侵染12种豆科和藜科植物。它在豇豆上引起系统花叶、叶片卷曲、明脉和畸形等症状。它在苋色藜、昆诺藜和蚕豆上表现为局部病斑。体外抗性测定,失毒温度55~60℃,稀释限点10^-3~10^-4,体外存活期1~2天。病毒极易摩擦接种传病。桃蚜、棉蚜和豆蚜都能传染这种病毒。人工接种的豇豆病株,在花器的各个部分、幼嫩的豆荚组织和末成熟的种子内都带有病毒。病株上采收的种子传毒率可达8.1%。病毒存在于种子的胚和子叶内,种皮内没有测到病毒。病毒粒体线条状,长700~750纤米。病株叶片表皮细胞内有纺锤状的内含体。免疫电镜和SDS~双扩散法测定,病毒分离物C-1与豇豆蚜传花叶病毒(CAMV)的抗血清呈阳性反应。根据以上这些性状,病毒分离物C-1可鉴定为属于马铃薯Y病毒组中的豇豆蚜传花叶病毒。用微量沉淀法测定,病毒粗提纯液制备的抗血清的效价为1:512。SDS-双扩散法测定,南京地区严重发生的豇豆花叶病中,85~86%是由豇豆蚜传花叶病毒引起的。从福建、山东、辽宁等省采集的样本中,也证实这种病毒在豇豆上普遍发生。

IDENTIFICATION OF COWPEA APHID-BORNE MOSAIC VIRUS IN NANJING

The C-1 isolate was isolated from asparagus bean(Vigna sesquipedalis) in Nanjing.The isolate could infects 12 species of plants in Leguminosea and Chenopodiaceae,causing local lesions in Chenopodium amaranticolor,C.quinoa and Vicia faba,and systemical infection with deformation and down-rolled leaf in asparagus bean.In crude sap of asparagus bean,the thermal inactivation point was 55~60℃,dilution end point 10-3~10-4,and longevity in vitro 1~2 days.The virus was readily transmitted by sap inoculation,and by Myzus persicae,Aphis gossypii and A.craccivora in non-persistent manner.The virus was seed-transmitted in the asparagus bean at a percentage of 8.1.The filamentous particles about 700~750nm in length were observed under the electro microscopy.The spindle-like inclusion bodies appeared in the epidermal cells of infected leaf.The virus could react with cowpea aphid-borne mosaic virus(CAMV) antiserum in ISEM and SDS-immunodiffusion tests.On the basis of these characteri-stics,the authors consider the isolate as CAMV and belongs to the Po-tyvirus group.