| 苹果IRAP技术体系的建立及优化 |
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| 引用本文： | 贾怀志,刘艳红,渠慎春,高志红,王昆,章镇.苹果IRAP技术体系的建立及优化[J].果树学报,2009,26(2). |
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| 作者姓名： | 贾怀志 刘艳红 渠慎春 高志红 王昆 章镇 |
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| 作者单位： | 贾怀志,刘艳红,渠慎春,高志红,章镇,JIA Huai-zhi,LIU Yan-hong,QU Shen-chun,GAO Zhi-hong,ZHANG Zhen(南京农业大学园艺学院,南京,210095);王昆,WANG kun(中国农业科学院果树研究所,辽宁兴城,125100)
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| 摘 要： | 通过苹果逆转座子长末端重复序列(LTR)保守区域设计引物,对影响苹果IRAP反应的重要因素进行了研究,建立并优化了苹果IRAP分子标记技术体系。优化的苹果IRAP分子标记体系为:25μL反应体系中,模板DNA50ng、Mg2+2.5mmol·L-1、dNTPs0.2mmol·L-1、10×buffer2.5mmol·L-1、TaqDNA聚合酶1.0U、引物0.4μmol·L-1。该体系在所试苹果品种中均获得了较理想的扩增效果,并能鉴定部分芽变品种。
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| 关 键 词： | 苹果 逆转座子 IRAP 技术体系 |
Establishment and optimization of the inter -retrotransposon amplified polymorphism(IRAP) molecular marker system in apple |
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| JIA Huai-zhi,LIU Yan-hong,QU Shen-chun,GAO Zhi-hong,WANG kun,ZHANG Zhen.Establishment and optimization of the inter -retrotransposon amplified polymorphism(IRAP) molecular marker system in apple[J].Journal of Fruit Science,2009,26(2). |
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| Authors: | JIA Huai-zhi LIU Yan-hong QU Shen-chun GAO Zhi-hong WANG kun ZHANG Zhen |
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| Institution: | 1College of Horticulture;Nanjing Agticultural University;Nanjing;Jiangsu 210095 China;2Research Institute of Pomology;CAAS;xingcheng;Liaoning 125100 China |
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| Abstract: | IRAP primers were designed based on Long Terminal Repeat(LTR) reserve region of retrotransposon in apple.Several important reaction factors of IRAP were studied in order to establish and optimize IRAP system in apple.The final optimal system was established as follows:template DNA 50 ng,Mg2+ 2.5 mmol·L-1、dNTP 0.2 mmol·L-1,10×buffer 2.5 mmol· L-1,Taq DNA polymerase 1.0 U,primer 0.4 μmol·L-1 in 25 μL reaction system.Ideal amplification results were obtained by using such IRAP system in tested apple cultivars,especially in identifying the bud mutation. |
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| Keywords: | IRAP |
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