樱桃主栽品种的遗传多样性分析

利用24对SSR引物对30个樱桃主栽品种进行遗传多样性分析, 以了解樱桃产区的资源多样性状况, 为种质资源的开发和利用提供帮助。结果表明: 樱桃产区具有丰富的遗传变异。SSR的遗传多样性指数的分布范围为1.3647～2.9964, Simp son指数分布范围为0.6111～0.9326。30个品种的分子数据聚类结果均呈现一定的地理分布规律。根据系统聚类分析将30个樱桃品种分为两大类: 欧洲甜樱桃和中国樱桃, 与传统分类学的结果相符。同时在两大类内又将品种进行细分, 第Ⅰ类分为3组, 第Ⅱ类分为2组,其结果与地理分布有一定的相关性, 能够反映樱桃的遗传特点及区域特性。

Analysis of Genetic Diversity in Cerasus by SSR Markers

The genetic diversity of thirty cultivars of Prunus avium L. and P. pseudocerasus Lindl. were evaluated by 24 pairs of SSR primers. The aim of the study is to reveal the genetic diversity in Cerasus and help to exploit and make use of the germplasm of Cerasus. The results showed that the Shannon-weaver index of genetic diversity ranged from 1.3647 to 2.9964 and the Simpson index from 0.6111 to 0.9326, which indicated that the Cerasus has abundant genetic diversity. The molecular clustering showed some rules in the geographical distribution. The cultivars used in this study were divided into 2 groups by cluster analysis: P. avium L and P. pseudocerasus Lindl., which was in agreement with the traditional taxonomy on Cerasus. Moreover, P. avium L. was subdivided into 3 subgroups, and P. pseudocerasus Lindl. into 2 subgroups respectively. The cluster results showed that the molecular classification of Cerasus in China had some relativity to the geographic taxonomic system, which reflected the genetic and territorial character of Cerasus.